SFRP5 is a target gene transcriptionally regulated by PPARγ in 3T3-L1 adipocytes.

Secreted frizzled-related protein 5 (SFRP5) is a newly identified adipokine. SFRP5 expression increases during the differentiation and maturation of adipocytes, but the factors regulating SFRP5 expression during this process remain unclear. This study showed that peroxisome proliferator-activated receptor γ (PPARγ) adenovirus transfection could enhance the SFRP5 expression of 3T3-L1 adipocytes. Three potential binding sites of PPARγ in the SFRP5 promoter domain were found by bioinformatics analysis. Luciferase reporter gene assay demonstrated that PPARγ regulated the activity of the SFRP5 promoter through cis-acting elements at -2,284--1,500bp. Further experiments verified that PPARγ could specifically bind to the SFRP5 promoter at -2,284--2,263bp using chromatin immunoprecipitation and electrophoretic mobility shift assay. These results suggest that SFRP5 be a target gene of PPARγ, and its expression may be under the transcriptional regulation of PPARγ.