| Literature DB >> 29066034 |
Florencia Rebaque1, Pablo Camacho2, Julián Parada2, Paula Lucchesi3, Arnaldo Ambrogi1, Pablo Tamiozzo4.
Abstract
Two cross-sectional studies were carried out in 2013 and 2015 monitoring for Mycoplasma hyopneumoniae presence in a swine farm. In these studies, the genetic diversity of M. hyopneumoniae was assessed in clinical specimens using a Multiple Locus Variable-number tandem repeat Analysis (MLVA) targeting P97 R1, P146 R3 and H4 loci. The samples from August 2015 showed the MLVA profile prevalent in June 2013, therefore it can be concluded that a same genetic type of M. hyopneumoniae can persist for at least two years in a closed herd. In addition, the nested PCR reactions implemented in this study showed to be useful for MLVA typing in non-invasive clinical samples.Entities:
Keywords: Análisis Multilocus de Regiones Repetidas en Tándem; Cerdo; Multiple Locus Variable-number tandem repeat Analysis; Mycoplasma hyopneumoniae; Persistence; Persistencia; Polymerase chain reaction; Reacción en cadena de la polimerasa; Swine; Tipificación; Typing
Mesh：
Year: 2017 PMID： 29066034 DOI： 10.1016/j.ram.2017.05.002
Source DB: PubMed Journal: Rev Argent Microbiol ISSN： 0325-7541 Impact factor: 1.852