Kazuyo Nishikawa1,2, Fumiaki Itoi3, Miki Nagahara4, Mami Jose4, Ayumi Matsunaga4, Jun Ueda5,6, Takashi Iwamoto4. 1. Department of Infertility, Green Bell Clinic, Kitamachi 2-160, Toyota, Aichi, 471-0027, Japan. 2. Department of Infertility, Royal Bell Clinic, Midori-ku, Nagoya, Aichi, 458-0801, Japan. 3. Department of Infertility, Green Bell Clinic, Kitamachi 2-160, Toyota, Aichi, 471-0027, Japan. fumiaki-itoi@kishokai.or.jp. 4. Center for Education in Laboratory Animal Research, Chubu University, Kasugai, Aichi, 487-8501, Japan. 5. Center for Education in Laboratory Animal Research, Chubu University, Kasugai, Aichi, 487-8501, Japan. junueda@asahikawa-med.ac.jp. 6. Center for Advanced Research and Education, Asahikawa Medical University, Asahikawa, Hokkaido, 078-8510, Japan. junueda@asahikawa-med.ac.jp.
Abstract
PURPOSE: The purpose of this report is to analyze the chromosome status and fertilization capability of sperm obtained from an infertile male patient with ring chromosome 15. METHODS: This was a case report at a private in vitro fertilization clinic. A man diagnosed with severe oligozoospermia carrying ring chromosome 15. To evaluate the chromosome status and fertilization capability, sperm from a patient carrying ring chromosome 15 were injected into enucleated mouse oocytes. RESULTS: The karyotypes of motile sperm from a patient carrying ring chromosome 15 were normal, and ring chromosome 15 was not observed in the chromosome spread samples of 1PN. In addition, these motile sperm retained the fertilization capability. However, the fertilization rates decreased (85.2, 76.2, and 64.3%, respectively) along with the decline of the aspect ratio of the sperm head (≥ 1.50, 1.30-1.49, and < 1.30, respectively). CONCLUSIONS: The karyotypes were normal without ring chromosome 15, and motile sperm with a high aspect ratio showed adequate potential for fertilization.
PURPOSE: The purpose of this report is to analyze the chromosome status and fertilization capability of sperm obtained from an infertile male patient with ring chromosome 15. METHODS: This was a case report at a private in vitro fertilization clinic. A man diagnosed with severe oligozoospermia carrying ring chromosome 15. To evaluate the chromosome status and fertilization capability, sperm from a patient carrying ring chromosome 15 were injected into enucleated mouse oocytes. RESULTS: The karyotypes of motile sperm from a patient carrying ring chromosome 15 were normal, and ring chromosome 15 was not observed in the chromosome spread samples of 1PN. In addition, these motile sperm retained the fertilization capability. However, the fertilization rates decreased (85.2, 76.2, and 64.3%, respectively) along with the decline of the aspect ratio of the sperm head (≥ 1.50, 1.30-1.49, and < 1.30, respectively). CONCLUSIONS: The karyotypes were normal without ring chromosome 15, and motile sperm with a high aspect ratio showed adequate potential for fertilization.
Authors: Laura K Conlin; Whitney Kramer; Anne L Hutchinson; Xia Li; Harold Riethman; Hakon Hakonarson; John C Mulley; Ingrid E Scheffer; Samuel F Berkovic; Syed A Hosain; Nancy B Spinner Journal: J Med Genet Date: 2010-10-23 Impact factor: 6.318
Authors: César Paz-Y-Miño; Jaime Guevara-Aguirre; Ariane Paz-Y-Miño; Francesca Velarde; Isaac Armendáriz-Castillo; Verónica Yumiceba; Jesús María Hernández; Juan Luis García; Paola E Leone Journal: J Med Case Rep Date: 2018-11-16