Li Wang1, Peng Li2. 1. Department of Medicine Technology Optometry, Xi'an Medical College, Xi'an 710021, Shaanxi Province, China. 2. Department of Ophthalmology, No.451 Hospitial of Chinese PLA, Xi'an 710054, Shaanxi Province, China.
Abstract
AIM: To assess the expression of nestin and glial fibrillary acidic protein (GFAP) in rat retina after optic nerve transection. METHODS: Rats were randomly divided into normal control group, sham group and operation group, and used for establishing an animal model of optic nerve transection. Retinal specimen of each group was collected at 3, 48h, 7 and 14d postoperative. Nestin and GFAP expressions on sagittal sections were analyzed by immunohistochemical staining, and protein extraction was analyzed by Western blot. RESULTS: Immunohistochemical analysis showed that nestin positive staining was rarely detected in normal control group and sham group, while sham group showed weak positive staining at 3h postoperative, the reaction gradually increased at 48h postoperative, and reached its maximum at 7d postoperative, and then decreased at 14d postoperative. Compared to the expression of GFAP, there was not statistically significant obvious difference among three groups (P>0.05). Result of Western blot method was consistent with that of immunohistochemical method. CONCLUSION: The expression of nestin increased in a time dependent fashion in Müller cells of retina following optic nerve transection, which was statistically significant, but there was no obvious difference in GFAP expression. The results indicate that an increase in colloid synthesis in retina following optic nerve transection can improve the retinal neurons' environment.
AIM: To assess the expression of nestin and glial fibrillary acidic protein (GFAP) in rat retina after optic nerve transection. METHODS:Rats were randomly divided into normal control group, sham group and operation group, and used for establishing an animal model of optic nerve transection. Retinal specimen of each group was collected at 3, 48h, 7 and 14d postoperative. Nestin and GFAP expressions on sagittal sections were analyzed by immunohistochemical staining, and protein extraction was analyzed by Western blot. RESULTS: Immunohistochemical analysis showed that nestin positive staining was rarely detected in normal control group and sham group, while sham group showed weak positive staining at 3h postoperative, the reaction gradually increased at 48h postoperative, and reached its maximum at 7d postoperative, and then decreased at 14d postoperative. Compared to the expression of GFAP, there was not statistically significant obvious difference among three groups (P>0.05). Result of Western blot method was consistent with that of immunohistochemical method. CONCLUSION: The expression of nestin increased in a time dependent fashion in Müller cells of retina following optic nerve transection, which was statistically significant, but there was no obvious difference in GFAP expression. The results indicate that an increase in colloid synthesis in retina following optic nerve transection can improve the retinal neurons' environment.
Authors: Ani V Das; Kavita B Mallya; Xing Zhao; Faraz Ahmad; Sumitra Bhattacharya; Wallace B Thoreson; Ganapati V Hegde; Iqbal Ahmad Journal: Dev Biol Date: 2006-07-29 Impact factor: 3.582
Authors: Elena Vecino; F David Rodriguez; Noelia Ruzafa; Xandra Pereiro; Sansar C Sharma Journal: Prog Retin Eye Res Date: 2015-06-23 Impact factor: 21.198
Authors: Nicolás Cuenca; Laura Fernández-Sánchez; Laura Campello; Victoria Maneu; Pedro De la Villa; Pedro Lax; Isabel Pinilla Journal: Prog Retin Eye Res Date: 2014-07-17 Impact factor: 21.198
Authors: Tasneem P Sharma; Colleen M McDowell; Yang Liu; Alex H Wagner; David Thole; Benjamin P Faga; Robert J Wordinger; Terry A Braun; Abbot F Clark Journal: Mol Neurodegener Date: 2014-04-27 Impact factor: 14.195
Authors: Craig S Pearson; Andrea G Solano; Sharada M Tilve; Caitlin P Mencio; Keith R Martin; Herbert M Geller Journal: Exp Eye Res Date: 2019-11-06 Impact factor: 3.467