Effect of growth conditions on β-glucosidase production using Flourensia cernua leaves in a solid-state fungal bioprocess.

Flourensia cernua foliage was used in a solid-state fungal bioprocess to identify factors that could affect β-glucosidase production such as growth medium components and partial identification of molecules from the plant material. Under an exploratory experimental design, each variable had their distinctive result on conditions, which affects and could further improve β-glucosidase production. Under the experimental design, 1482 U/L of β-glucosidase were detected, which marks an improvement in production compared to levels obtained in a control treatment with an activity of 1092 U/L. It was shown that inoculum, water content and pH were the factors with the greater effect on β-glucosidase production. Polyphenolic content and cellulosic fiber in the form of raw fiber were measured to assess compound degradation of the plant material. Although fiber content was apparently unaffected, polyphenolic content decreased; β-glucosidase was produced by A. niger GH1. This behavior could be associated with fiber level and polyphenolic content because molecules of this type can be hydrolyzed by β-glucosidase. According to our results, F. cernua biomass can be used as a carbon source for β-glucosidase production in a short culture time.