Gold Nanoparticles and Radio Frequency Field Interactions: Effects of Nanoparticle Size, Charge, Aggregation, Radio Frequency, and Ionic Background.

In this study, we investigated experimentally the dependency of radio frequency (rf) absorption by gold nanoparticles (AuNPs) on frequency (10 kHz to 450 MHz), NP size (3.5, 17, and 36 nm), charge of the ligand shell (positive amino and negative carboxylic functional groups), aggregation state, and presence of electrolytes (0-1 M NaCl). In addition, we examined the effect of protein corona on the rf absorption by AuNPs. For the first time, rf energy absorption by AuNPs was analyzed in the 10 kHz to 450 MHz rf range. We have demonstrated that the previously reported rf heating of AuNPs can be solely attributed to the heating of the ionic background and AuNPs do not absorb noticeable rf energy regardless of the NP size, charge, aggregation, and presence of electrolytes. However, the formation of protein corona on the AuNP surface resulted in rf energy absorption by AuNP-albumin constructs, suggesting that protein corona might be partially responsible for the heating of AuNPs observed in vivo. The optimal frequency of rf absorption for the AuNP-albumin constructs is significantly higher than conventional 13.56 MHz, suggesting that the heating of AuNPs in rf field should be performed at considerably higher frequencies for better results in vivo.