| Literature DB >> 29059222 |
Avishek Dey, Milan Kumar Samanta, Srimonta Gayen, Soumitra K Sen, Mrinal K Maiti.
Abstract
[This corrects the article DOI: 10.1371/journal.pone.0150763.].Entities:
Year: 2017 PMID: 29059222 PMCID: PMC5653364 DOI: 10.1371/journal.pone.0187172
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 6Assessing drought stress tolerance and grain yield of OsbZIP23 overexpression (OE) and down-regulated (RNAi) transgenic lines.
(A) Photographs of OE lines, RNAi lines and non-transgenic (NT) plants in the vegetative stage- before and after drought stress and their subsequent recovery after the drought treatment. (B) Survival rates of transgenic lines and NT plants, calculated in percentage (%). (C) Photographs of OE lines, RNAi lines and NT plants in early reproductive (panicle initiation) stage- before and after drought stress and their subsequent recovery after the drought treatment. (D) Survival rates of transgenic lines and NT plants, calculated in %. (E) Drought stress in PVC pipes in flowering stage and subsequent recovery till seed maturation stage of OE lines, RNAi lines and NT plants. (F) Mature panicle of OE lines, RNAi lines and NT plant. (G) Measurement of panicle weight in OE lines, RNAi lines and NT plant. (H) Spikelet fertility measurement in OE lines, RNAi lines and NT plants, calculated in %. Data bars represent the mean ±SD of triplicate measurement. Statistical analysis by Student’s t-test indicated significant differences (*P<0.05, ** P<0.01). All the results were based on three independent experiments.
Fig 9Evaluating ABA sensitivity of OsbZIP23 OE and RNAi lines at germination and post-germination stages.
Germination performance of seeds from (A) OsbZIP23 OE lines (OER#5, OEN#9), and (B) OsbZIP23 RNAi lines (RNAi#1, RNAi#4) in comparison to NT plants on MS agar medium containing 0, 1, 3 and 6 μM ABA at 10th day. (C) Calculation of the germination rates (%) of OsbZIP23 OE, RNAi and NT seeds. (D and E) Performance of OE, RNAi and NT seedlings in ½ MS liquid medium containing 0, 1, 3 and 6 μM of ABA. Measurement of (F) shoot length and (G) root length of OE, RNAi and NT seedlings grown on different concentrations of ABA after 14 days. For representation and better comparison, the NT panels are duplicated in A-B and D-E to represent the observed phenotype of NT plants, which was the same in all three replicate plates, examined in this study and was used to compare separately with the OE and RNAi lines in the respective panels. Data bars represent the mean ±SD of triplicate measurement. Statistical analysis by Student’s t-test indicated significant differences (*P<0.05, ** P<0.01). All the results were based on three independent experiments.