Endang S Kusmintarsih1, Rahma F Hayati2, Oktaviani N Turnip3, Benediktus Yohan2, Suhestri Suryaningsih1, Hery Pratiknyo1, Dionisius Denis2, R Tedjo Sasmono4. 1. Fakultas Biologi, Universitas Jenderal Soedirman, Jl. dr. Soeparno No. 63, Purwokerto, 53122, Indonesia. 2. Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta, 10430, Indonesia. 3. Fakultas Biologi, Universitas Jenderal Soedirman, Jl. dr. Soeparno No. 63, Purwokerto, 53122, Indonesia; Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta, 10430, Indonesia. 4. Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta, 10430, Indonesia. Electronic address: sasmono@eijkman.go.id.
Abstract
BACKGROUND: Dengue is hyper-endemic in Indonesia. Purwokerto city in Central Java province is routinely ravaged by the disease. Despite the endemicity of dengue in this city, there is still no data on the virological aspects of dengue in the city. We conducted a molecular surveillance study of the circulating dengue viruses (DENV) in Purwokerto city to gain information on the virus origin, serotype and genotype distribution, and phylogenetic characteristics of DENV. METHODS: A cross-sectional dengue molecular surveillance study was conducted in Purwokerto. Sera were collected from dengue-suspected patients attending three hospitals in the city. Diagnosis was performed using dengue NS1 antigen and IgG/IgM antibodies detection. DENV serotyping was performed using Simplexa Dengue real-time RT-PCR. Sequencing was conducted to obtain full-length DENV Envelope (E) gene sequences, which were then used in phylogenetic and genotypic analyses. Patients' clinical and demographic data were collected and analyzed. RESULTS: A total of 105 dengue-suspected patients' sera were collected, in which 80 (76.2%) were positive for IgM and/or IgG, and 57 (54.2%) were confirmed as dengue by NS1 antigen and/or DENV RNA detection using RT-PCR. Serotyping was successful for 47 isolates. All four serotypes circulated in the area with DENV-3 as the predominant serotype. Phylogenetic analyses grouped the isolates into Genotype I for DENV-1, Cosmopolitan genotype for DENV-2, and Genotype I and II for DENV-3 and -4, respectively. The analyses also revealed the close relatedness of Purwokerto isolates to other DENV strains from Indonesia and neighboring countries. CONCLUSION: We reveal the molecular and virological characteristics of DENV in Purwokerto, Banyumas regency, Central Java. The genotype and phylogenetic analyses indicate the endemicity of the circulating DENV in the city. Our serotype and genotype data provide references for future dengue molecular epidemiology studies and disease management in the region.
BACKGROUND: Dengue is hyper-endemic in Indonesia. Purwokerto city in Central Java province is routinely ravaged by the disease. Despite the endemicity of dengue in this city, there is still no data on the virological aspects of dengue in the city. We conducted a molecular surveillance study of the circulating dengue viruses (DENV) in Purwokerto city to gain information on the virus origin, serotype and genotype distribution, and phylogenetic characteristics of DENV. METHODS: A cross-sectional dengue molecular surveillance study was conducted in Purwokerto. Sera were collected from dengue-suspected patients attending three hospitals in the city. Diagnosis was performed using dengue NS1 antigen and IgG/IgM antibodies detection. DENV serotyping was performed using Simplexa Dengue real-time RT-PCR. Sequencing was conducted to obtain full-length DENV Envelope (E) gene sequences, which were then used in phylogenetic and genotypic analyses. Patients' clinical and demographic data were collected and analyzed. RESULTS: A total of 105 dengue-suspected patients' sera were collected, in which 80 (76.2%) were positive for IgM and/or IgG, and 57 (54.2%) were confirmed as dengue by NS1 antigen and/or DENV RNA detection using RT-PCR. Serotyping was successful for 47 isolates. All four serotypes circulated in the area with DENV-3 as the predominant serotype. Phylogenetic analyses grouped the isolates into Genotype I for DENV-1, Cosmopolitan genotype for DENV-2, and Genotype I and II for DENV-3 and -4, respectively. The analyses also revealed the close relatedness of Purwokerto isolates to other DENV strains from Indonesia and neighboring countries. CONCLUSION: We reveal the molecular and virological characteristics of DENV in Purwokerto, Banyumas regency, Central Java. The genotype and phylogenetic analyses indicate the endemicity of the circulating DENV in the city. Our serotype and genotype data provide references for future dengue molecular epidemiology studies and disease management in the region.
Authors: I Made Susila Utama; Nurhayati Lukman; Dewi Dian Sukmawati; Bachti Alisjahbana; Anggraini Alam; Dewi Murniati; I Made Gede Dwi Lingga Utama; Dwiyanti Puspitasari; Herman Kosasih; Ida Laksono; Muhammad Karyana; Mulya Rahma Karyanti; M M D E A H Hapsari; Ninny Meutia; C Jason Liang; Wahyu Nawang Wulan; Chuen-Yen Lau; Ketut Tuti Merati Parwati Journal: PLoS Negl Trop Dis Date: 2019-10-21
Authors: Aryati Aryati; Billy J Wrahatnala; Benediktus Yohan; May Fanny; Faradila K N Hakim; Eka Putri Sunari; Nelly Zuroidah; Puspa Wardhani; Marsha S Santoso; Dominicus Husada; Ali Rohman; Siti Nadia Tarmizi; Justus T O Sievers; R Tedjo Sasmono Journal: Viruses Date: 2020-08-20 Impact factor: 5.048
Authors: Emma J Quinn; Allena H-C Cheong; Julie K Calvert; Geoffrey Higgins; Trish Hahesy; David L Gordon; Jillian M Carr Journal: Trop Med Infect Dis Date: 2018-01-07