F Cipriani1,2, C Mastrorilli1,3, S Tripodi4, G Ricci2, S Perna1, V Panetta5, R Asero6, A Dondi7, A Bianchi8, N Maiello9, M Miraglia Del Giudice9, T Frediani10, F Macrì10, S Lucarelli10, I Dello Iacono11, M F Patria12, E Varin13, D Peroni14, L Chini15, V Moschese15, R Bernardini16, G Pingitore17, U Pelosi18, M Tosca19, F Paravati20, I Sfika4, A Di Rienzo Businco4, C Povesi Dascola3, P Comberiati14, S Frediani10, C Lambiase10, M C Verga21, D Faggian22, M Plebani22, M Calvani8, C Caffarelli3, P M Matricardi1. 1. Department of Pediatric Pneumology and Immunology, Charité Medical University, Berlin, Germany. 2. Pediatric Unit, Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy. 3. Pediatric Clinic of Medicine and Surgery, University of Parma, Parma, Italy. 4. Pediatric Department and Pediatric Allergology Unit, Sandro Pertini Hospital, Rome, Italy. 5. Biostatistics, L'Altra Statistica srl, Consultancy & Training, Rome, Italy. 6. Allergology Service, San Carlo Clinic, Paderno Dugnano, Milan, Italy. 7. Pediatric Emergency Unit, S. Orsola-Malpighi Hospital, Bologna, Italy. 8. Pediatric Unit, San Camillo Forlanini Hospital, Rome, Italy. 9. Dipartimento della Donna, del Bambino e di Chirurgia Generale e Specialistica, Università della Campania Luigi Vanvitelli, Naples, Italy. 10. Pediatric Department, La Sapienza University, Rome, Italy. 11. Pediatric Unit, Fatebenefratelli Hospital, Benevento, Italy. 12. Pediatric Highly Intensive Care Unit, Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. 13. Pediatric Intermediate Care Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. 14. Pediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy. 15. Pediatric Allergology and Immunology Unit, Policlinico Tor Vergata, University of Rome Tor Vergata, Rome, Italy. 16. Pediatric Unit, San Giuseppe Hospital, Empoli, Italy. 17. Pediatric Unit, Grassi Hospital, Rome, Italy. 18. Pediatric Unit, Santa Barbara Hospital, Iglesias, Italy. 19. Pulmonary Disease and Allergy Unit, G. Gaslini Hospital, Genoa, Italy. 20. Pediatric Unit, Crotone, Italy. 21. Primary Care Pediatrics, ASL Salerno, Vietri sul Mare, Italy. 22. Department of Laboratory Medicine, University Hospital of Padua, Padua, Italy.
Abstract
BACKGROUND: Grass pollen-related seasonal allergic rhinoconjunctivitis (SARg) is clinically heterogeneous in severity, comorbidities, and response to treatment. The component-resolved diagnostics disclosed also a high heterogeneity at molecular level. Our study aimed at analyzing the characteristics of the IgE sensitization to Phleum pratense molecules and investigating the diagnostic relevance of such molecules in childhood. METHODS: We examined 1120 children (age 4-18 years) with SARg. Standardized questionnaires on atopy were acquired through informatics platform (AllergyCARD™). Skin prick tests were performed with pollen extracts. Serum IgE to airborne allergens and eight P. pratense molecules (rPhl p 1, rPhl p 2, rPhl p 4, rPhl p 5b, rPhl p 6, rPhl p 7, rPhl p 11, rPhl p 12) were tested by ImmunoCAP FEIA. RESULTS: The analysis of IgE responses against eight P. pratense molecules showed 87 profiles. According to the number of molecules recognized by IgE, the more complex profiles were characterized by higher serum total IgE, higher grass-specific serum IgE, and higher number and degree of sensitization to pollens. The most frequent IgE sensitization profile was the monomolecular Phl p 1. Sensitization to Phl p 7 was a reliable biomarker of asthma, whereas Phl p 12 of oral allergy syndrome. Sensitization to Phl p 7 was associated with a higher severity of SARg, and complex profiles were associated with longer disease duration. CONCLUSIONS: In a large pediatric population, the complexity of IgE sensitization profiles against P. pratense molecules is related to high atopic features although useless for predicting the clinical severity. The detection of serum IgE to Phl p 1, Phl p 7, and Phl p 12 can be used as clinical biomarkers of SARg and comorbidities. Further studies in different areas are required to test the impact of different IgE molecular profiles on AIT response.
BACKGROUND: Grass pollen-related seasonal allergic rhinoconjunctivitis (SARg) is clinically heterogeneous in severity, comorbidities, and response to treatment. The component-resolved diagnostics disclosed also a high heterogeneity at molecular level. Our study aimed at analyzing the characteristics of the IgE sensitization to Phleum pratense molecules and investigating the diagnostic relevance of such molecules in childhood. METHODS: We examined 1120 children (age 4-18 years) with SARg. Standardized questionnaires on atopy were acquired through informatics platform (AllergyCARD™). Skin prick tests were performed with pollen extracts. Serum IgE to airborne allergens and eight P. pratense molecules (rPhl p 1, rPhl p 2, rPhl p 4, rPhl p 5b, rPhl p 6, rPhl p 7, rPhl p 11, rPhl p 12) were tested by ImmunoCAP FEIA. RESULTS: The analysis of IgE responses against eight P. pratense molecules showed 87 profiles. According to the number of molecules recognized by IgE, the more complex profiles were characterized by higher serum total IgE, higher grass-specific serum IgE, and higher number and degree of sensitization to pollens. The most frequent IgE sensitization profile was the monomolecular Phl p 1. Sensitization to Phl p 7 was a reliable biomarker of asthma, whereas Phl p 12 of oral allergy syndrome. Sensitization to Phl p 7 was associated with a higher severity of SARg, and complex profiles were associated with longer disease duration. CONCLUSIONS: In a large pediatric population, the complexity of IgE sensitization profiles against P. pratense molecules is related to high atopic features although useless for predicting the clinical severity. The detection of serum IgE to Phl p 1, Phl p 7, and Phl p 12 can be used as clinical biomarkers of SARg and comorbidities. Further studies in different areas are required to test the impact of different IgE molecular profiles on AIT response.
Authors: Guillermo Til-Pérez; Claudio Carnevale; Pedro Luis Sarría-Echegaray; Diego Arancibia-Tagle; Sendy Chugo-Gordillo; Manuel David Tomás-Barberán Journal: Clin Mol Allergy Date: 2019-05-02
Authors: Maria Angela Tosca; Amelia Licari; Roberta Olcese; Riccardo Castagnoli; Alessia Marseglia; Gian Luigi Marseglia; Michele Miraglia Del Giudice; Alberto Martelli; Mauro Calvani; Carlo Caffarelli; Marzia Duse; Claudio Cravidi; Fabio Cardinale; Giorgio Ciprandi Journal: Acta Biomed Date: 2020-09-15
Authors: Michele Miraglia Del Giudice; Annalisa Allegorico; Gian Luigi Marseglia; Alberto Martelli; Mauro Calvani; Fabio Cardinale; Marzia Duse; Elena Chiappini; Sara Manti; Claudio Cravidi; Maria Angela Tosca; Carlo Caffarelli Journal: Acta Biomed Date: 2020-09-15