Literature DB >> 29048971

Protocol for Efficient Generation and Characterization of Adeno-Associated Viral Vectors.

Andreas Jungmann1, Barbara Leuchs2, Jean Rommelaere2, Hugo A Katus1, Oliver J Müller1,3.   

Abstract

Adeno-associated virus vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high adeno-associated virus titers within 6 working days. The same procedure can be used for all serotypes and thus allows direct comparability of different serotypes. In this protocol we describe a step-by-step procedure that results in well-characterized vectors suitable for both in vitro approaches and preclinical studies.

Entities:  

Keywords:  adeno-associated virus (AAV); quality control; titration; vector purification; viral vector

Mesh：

Year:  2017        PMID： 29048971     DOI： 10.1089/hgtb.2017.192

Source DB:  PubMed          Journal:  Hum Gene Ther Methods        ISSN： 1946-6536            Impact factor:   2.396


  21 in total

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Journal:  Elife       Date:  2018-08-23       Impact factor: 8.140

2.  Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors.

Authors:  Kyle B Fischer; Hannah K Collins; Edward M Callaway
Journal:  Proc Natl Acad Sci U S A       Date:  2019-12-16       Impact factor: 11.205

3.  AKAP18δ Anchors and Regulates CaMKII Activity at Phospholamban-SERCA2 and RYR.

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Journal:  Circ Res       Date:  2021-11-24       Impact factor: 23.213

4.  Liver alanine catabolism promotes skeletal muscle atrophy and hyperglycaemia in type 2 diabetes.

Authors:  Jürgen G Okun; Patricia M Rusu; Andrea Y Chan; Yuqin Wu; Yann W Yap; Thomas Sharkie; Jonas Schumacher; Kathrin V Schmidt; Katherine M Roberts-Thomson; Ryan D Russell; Annika Zota; Susanne Hille; Andreas Jungmann; Ludovico Maggi; Young Lee; Matthias Blüher; Stephan Herzig; Michelle A Keske; Mathias Heikenwalder; Oliver J Müller; Adam J Rose
Journal:  Nat Metab       Date:  2021-03-18

5.  Ligand-activated RXFP1 gene therapy ameliorates pressure overload-induced cardiac dysfunction.

Authors:  Nuttarak Sasipong; Philipp Schlegel; Julia Wingert; Christoph Lederer; Eric Meinhardt; Amelie Ziefer; Constanze Schmidt; Kleopatra Rapti; Cornelia Thöni; Norbert Frey; Patrick Most; Hugo A Katus; Philip W J Raake
Journal:  Mol Ther       Date:  2021-04-09       Impact factor: 12.910

6.  High-throughput mapping of a whole rhesus monkey brain at micrometer resolution.

Authors:  Fang Xu; Yan Shen; Lufeng Ding; Chao-Yu Yang; Heng Tan; Hao Wang; Qingyuan Zhu; Rui Xu; Fengyi Wu; Yanyang Xiao; Cheng Xu; Qianwei Li; Peng Su; Li I Zhang; Hong-Wei Dong; Robert Desimone; Fuqiang Xu; Xintian Hu; Pak-Ming Lau; Guo-Qiang Bi
Journal:  Nat Biotechnol       Date:  2021-07-26       Impact factor: 68.164

7.  The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4.

Authors:  Zegeye H Jebessa; Kumar D Shanmukha; Matthias Dewenter; Lorenz H Lehmann; Chang Xu; Friederike Schreiter; Dominik Siede; Xue-Min Gong; Barbara C Worst; Giuseppina Federico; Sven W Sauer; Tamas Fischer; Lisa Wechselberger; Oliver J Müller; Samuel Sossalla; Christoph Dieterich; Patrick Most; Herrmann-Josef Gröne; Cedric Moro; Monika Oberer; Guenter Haemmerle; Hugo A Katus; Jens Tyedmers; Johannes Backs
Journal:  Nat Metab       Date:  2019-11-15

8.  The E3 ubiquitin ligase HectD3 attenuates cardiac hypertrophy and inflammation in mice.

Authors:  Ashraf Yusuf Rangrez; Ankush Borlepawar; Nesrin Schmiedel; Anushka Deshpande; Anca Remes; Manju Kumari; Alexander Bernt; Lynn Christen; Andreas Helbig; Andreas Jungmann; Samuel Sossalla; Andreas Tholey; Oliver J Müller; Derk Frank; Norbert Frey
Journal:  Commun Biol       Date:  2020-10-09

9.  Restriction of essential amino acids dictates the systemic metabolic response to dietary protein dilution.

Authors:  Yann W Yap; Patricia M Rusu; Andrea Y Chan; Barbara C Fam; Andreas Jungmann; Samantha M Solon-Biet; Christopher K Barlow; Darren J Creek; Cheng Huang; Ralf B Schittenhelm; Bruce Morgan; Dieter Schmoll; Bente Kiens; Matthew D W Piper; Mathias Heikenwälder; Stephen J Simpson; Stefan Bröer; Sofianos Andrikopoulos; Oliver J Müller; Adam J Rose
Journal:  Nat Commun       Date:  2020-06-09       Impact factor: 14.919

10.  Preclinical evidence for the therapeutic value of TBX5 normalization in arrhythmia control.

Authors:  Franziska S Rathjens; Alica Blenkle; Lavanya M Iyer; Anke Renger; Fahima Syeda; Claudia Noack; Andreas Jungmann; Matthias Dewenter; Karl Toischer; Ali El-Armouche; Oliver J Müller; Larissa Fabritz; Wolfram-Hubertus Zimmermann; Laura C Zelarayan; Maria-Patapia Zafeiriou
Journal:  Cardiovasc Res       Date:  2021-07-07       Impact factor: 10.787
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