| Literature DB >> 29043634 |
Clinton D Orebaugh1, Scott A Lujan1, Adam B Burkholder2, Anders R Clausen3, Thomas A Kunkel4.
Abstract
Ribonucleotides embedded within DNA render the DNA sensitive to the formation of single-stranded breaks under alkali conditions. Here, we describe a next-generation sequencing method called hydrolytic end sequencing (HydEn-seq) to map ribonucleotides inserted into the genome of Saccharomyce cerevisiae strains deficient in ribonucleotide excision repair. We use this method to map several genomic features in wild-type and replicase variant yeast strains.Entities:
Keywords: Bioinformatics; DNA polymerase; DNA repair; DNA replication; Genomics; HydEn-seq; Next-generation sequencing; RNase H2; Ribonucleotide excision repair
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Year: 2018 PMID： 29043634 DOI： 10.1007/978-1-4939-7306-4_23
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745