| Literature DB >> 2903845 |
Abstract
This paper describes a method for isolation of DNA from blood samples involving a rapid chemical disintegration of proteins with 8 M urea and with a minimum of exposure to phenol. The DNA is further desalted and purified on Sephadex G-25 prepacked disposable columns. DNA isolated in this way was pure enough to be immediately cleaved by restriction enzymes.Entities:
Mesh:
Substances:
Year: 1988 PMID: 2903845 DOI: 10.1016/0735-0651(88)90003-9
Source DB: PubMed Journal: Gene Anal Tech ISSN: 0735-0651