Literature DB >> 2903843

Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer.

J Hanish1, M McClelland.   

Abstract

A single buffer consisting primarily of potassium and glutamate is shown to be suitable for activity of almost all restriction endonucleases, DNA methylases, and many other DNA-modifying enzymes. The composition of this buffer differs substantially from buffers currently in use for these enzymes.

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Year:  1988        PMID： 2903843     DOI： 10.1016/0735-0651(88)90005-2

Source DB:  PubMed          Journal:  Gene Anal Tech        ISSN： 0735-0651


  17 in total

1.  The FokI methyltransferase from Flavobacterium okeanokoites. Purification and characterization of the enzyme and its truncated derivatives.

Authors:  T Kaczorowski; M Sektas; P Skowron; A J Podhajska
Journal:  Mol Biotechnol       Date:  1999-11       Impact factor: 2.695

2.  A BlnI restriction map of the Salmonella typhimurium LT2 genome.

Authors:  K K Wong; M McClelland
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490

3.  Characteristics of Insertional Mutants of Pseudomonas syringae with Reduced Epiphytic Fitness.

Authors:  S E Lindow; G Andersen; G A Beattie
Journal:  Appl Environ Microbiol       Date:  1993-05       Impact factor: 4.792

4.  Methylase-limited partial NotI cleavage for physical mapping of genomic DNA.

Authors:  J Hanish; M McClelland
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

5.  Methylation at overlapping dam (Gm6ATC) sites does not block cleavage by the NruI (TCGCGA) isoschizomer restriction endonucleases AmaI, SalDI and Sbo13I.

Authors:  Y Patel; M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

6.  Sequencing, chromosomal inactivation, and functional expression in Escherichia coli of ppsR, a gene which represses carotenoid and bacteriochlorophyll synthesis in Rhodobacter sphaeroides.

Authors:  R J Penfold; J M Pemberton
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

7.  Genetic organization and molecular analysis of the EcoVIII restriction-modification system of Escherichia coli E1585-68 and its comparison with isospecific homologs.

Authors:  Iwona Mruk; Tadeusz Kaczorowski
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

8.  Stress-inducible gene of Salmonella typhimurium identified by arbitrarily primed PCR of RNA.

Authors:  K K Wong; M McClelland
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

9.  Reversible inactivation of a foreign gene, hph, during the asexual cycle in Neurospora crassa transformants.

Authors:  N N Pandit; V E Russo
Journal:  Mol Gen Genet       Date:  1992-09

10.  The occurrence of the transposable element pogo in Drosophila melanogaster.

Authors:  I A Boussy; L Charles; M H Hamelin; G Periquet; D Y Shapiro
Journal:  Genetica       Date:  1993       Impact factor: 1.082
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