Oya Sayin1, Serap Cilaker Micili2, Asli Goker3, Gonca Kamaci4, Bekir Ugur Ergur2, Osman Yilmaz4, Gul Guner Akdogan5. 1. Research Laboratory, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey. Electronic address: oya.sayin@deu.edu.tr. 2. Department of Histology and Embryology, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey. 3. Department of Obstetrics and Gynecology, Celal Bayar University, Faculty of Medicine, Manisa, Turkey. 4. Department of Laboratory Animal Science, Dokuz Eylul University, Faculty of Medicine, Izmir, Turkey. 5. Health Science Institute, Izmir University of Economics, Izmir, Turkey.
Abstract
OBJECTIVE: Healing of the uterus after cesarean section and myomectomy operation is clinically important. In this study, we aimed to investigate the effects of resveratrol (3,5,4'-o-trihydroxystilbene) on the wound healing process of the uterus in rats treated with resveratrol following full thickness injury of the uterus. MATERIALS AND METHODS: Twenty-one female wistar albino rats were divided randomly into three groups (1) control group with no intervention (2) injury group with uterine full thickness injury (3) resveratrol group with uterine full thickness injury and treated with resveratrol. Resveratrol was injected by oral gavage at the doses of 0.5 mg/kg/day for 30 days following uterine full thickness injury. Vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) distributions were assessed using the immunohistochemical methods in tissue and ELISA methods in the tissue homogenate. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were evaluated with colorimetric method and malondialdehyde (MDA) levels also were measured using high performance liquid chromatography in the tissue homogenate. The effects of resveratrol on the uterine histology also were evaluated histologically with the light microscopy. RESULTS: Histological evaluation and immunohistochemical evaluations showed that treatment with a resveratrol significantly increased the thickness of the uterine wall and VEGF expression and decreased expression PDGF during wound healing. Biochemically, GPx and SOD activities were increased significantly after treatment with resveratrol. Additionally, resveratrol administration decreased MDA levels. CONCLUSION: These results showed that the antioxidant effects of resveratrol has been shown to have a positive influence on wound healing of the uterus.
OBJECTIVE: Healing of the uterus after cesarean section and myomectomy operation is clinically important. In this study, we aimed to investigate the effects of resveratrol (3,5,4'-o-trihydroxystilbene) on the wound healing process of the uterus in rats treated with resveratrol following full thickness injury of the uterus. MATERIALS AND METHODS: Twenty-one female wistar albino rats were divided randomly into three groups (1) control group with no intervention (2) injury group with uterine full thickness injury (3) resveratrol group with uterine full thickness injury and treated with resveratrol. Resveratrol was injected by oral gavage at the doses of 0.5 mg/kg/day for 30 days following uterine full thickness injury. Vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) distributions were assessed using the immunohistochemical methods in tissue and ELISA methods in the tissue homogenate. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were evaluated with colorimetric method and malondialdehyde (MDA) levels also were measured using high performance liquid chromatography in the tissue homogenate. The effects of resveratrol on the uterine histology also were evaluated histologically with the light microscopy. RESULTS: Histological evaluation and immunohistochemical evaluations showed that treatment with a resveratrol significantly increased the thickness of the uterine wall and VEGF expression and decreased expression PDGF during wound healing. Biochemically, GPx and SOD activities were increased significantly after treatment with resveratrol. Additionally, resveratrol administration decreased MDA levels. CONCLUSION: These results showed that the antioxidant effects of resveratrol has been shown to have a positive influence on wound healing of the uterus.
Authors: N B Tikhonova; A P Milovanov; V V Aleksankina; T V Fokina; M N Boltovskaya; A P Aleksankin; K A Artem'eva Journal: Bull Exp Biol Med Date: 2021-11-17 Impact factor: 0.804