Literature DB >> 29037139

Analysis of Free Amino Acids in Mammalian Brain Extracts.

A L Ksenofontov1, A I Boyko, G V Mkrtchyan, V N Tashlitsky, A V Timofeeva, A V Graf, V I Bunik, L A Baratova.   

Abstract

An optimized method for analysis of free amino acids using a modified lithium-citrate buffer system with a Hitachi L-8800 amino acid analyzer is described. It demonstrates clear advantages over the sodium-citrate buffer system commonly used for the analysis of protein hydrolysates. A sample pretreatment technique for amino acid analysis of brain extracts is also discussed. The focus has been placed on the possibility of quantitative determination of the reduced form of glutathione (GSH) with simultaneous analysis of all other amino acids in brain extracts. The method was validated and calibration coefficient (KGSH) was determined. Examples of chromatographic separation of free amino acids in extracts derived from different parts of the brain are presented.

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Year:  2017        PMID： 29037139     DOI： 10.1134/S000629791710011X

Source DB:  PubMed          Journal:  Biochemistry (Mosc)        ISSN： 0006-2979            Impact factor:   2.487


  13 in total

1.  Administration of Phosphonate Inhibitors of Dehydrogenases of 2-Oxoglutarate and 2-Oxoadipate to Rats Elicits Target-Specific Metabolic and Physiological Responses.

Authors:  Victoria I Bunik; Artem V Artiukhov; Alexey V Kazantsev; Vasily A Aleshin; Alexandra I Boyko; Alexander L Ksenofontov; Nikolay V Lukashev; Anastasia V Graf
Journal:  Front Chem       Date:  2022-06-20       Impact factor: 5.545

2.  Delayed Impact of 2-Oxoadipate Dehydrogenase Inhibition on the Rat Brain Metabolism Is Linked to Protein Glutarylation.

Authors:  Alexandra I Boyko; Irina S Karlina; Lev G Zavileyskiy; Vasily A Aleshin; Artem V Artiukhov; Thilo Kaehne; Alexander L Ksenofontov; Sergey I Ryabov; Anastasia V Graf; Angela Tramonti; Victoria I Bunik
Journal:  Front Med (Lausanne)       Date:  2022-06-01

3.  Acid Hydrolyzed Silk Peptide Consumption Improves Anti-Diabetic Symptoms by Potentiating Insulin Secretion and Preventing Gut Microbiome Dysbiosis in Non-Obese Type 2 Diabetic Animals.

Authors:  Sunmin Park; Ting Zhang; Jing Yi Qiu; Xuangao Wu; Jeong-Yong Lee; Boo-Yong Lee
Journal:  Nutrients       Date:  2020-01-24       Impact factor: 5.717

4.  Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy.

Authors:  Anastasia Graf; Lidia Trofimova; Alexander Ksenofontov; Lyudmila Baratova; Victoria Bunik
Journal:  Cells       Date:  2020-01-07       Impact factor: 6.600

5.  Severe Spinal Cord Injury in Rats Induces Chronic Changes in the Spinal Cord and Cerebral Cortex Metabolism, Adjusted by Thiamine That Improves Locomotor Performance.

Authors:  Alexandra Boyko; Polina Tsepkova; Vasily Aleshin; Artem Artiukhov; Garik Mkrtchyan; Alexander Ksenofontov; Lyudmila Baratova; Sergey Ryabov; Anastasia Graf; Victoria Bunik
Journal:  Front Mol Neurosci       Date:  2021-03-29       Impact factor: 5.639

6.  Inhibition of 2-Oxoglutarate Dehydrogenase as a Chemical Model of Acute Hypobaric Hypoxia.

Authors:  Anastasia Graf; Alexander Ksenofontov; Victoria Bunik
Journal:  Front Med (Lausanne)       Date:  2021-12-17

7.  Increasing Inhibition of the Rat Brain 2-Oxoglutarate Dehydrogenase Decreases Glutathione Redox State, Elevating Anxiety and Perturbing Stress Adaptation.

Authors:  Artem V Artiukhov; Anastasia V Graf; Alexey V Kazantsev; Alexandra I Boyko; Vasily A Aleshin; Alexander L Ksenofontov; Victoria I Bunik
Journal:  Pharmaceuticals (Basel)       Date:  2022-01-31

8.  Delayed Influence of Spinal Cord Injury on the Amino Acids of NO• Metabolism in Rat Cerebral Cortex Is Attenuated by Thiamine.

Authors:  Alexandra Boyko; Alexander Ksenofontov; Sergey Ryabov; Lyudmila Baratova; Anastasia Graf; Victoria Bunik
Journal:  Front Med (Lausanne)       Date:  2018-01-15

9.  Activation of Mitochondrial 2-Oxoglutarate Dehydrogenase by Cocarboxylase in Human Lung Adenocarcinoma Cells A549 Is p53/p21-Dependent and Impairs Cellular Redox State, Mimicking the Cisplatin Action.

Authors:  Victoria I Bunik; Vasily A Aleshin; Xiaoshan Zhou; Vyacheslav Yu Tabakov; Anna Karlsson
Journal:  Int J Mol Sci       Date:  2020-05-26       Impact factor: 5.923

10.  Using enzymatic hydrolyzate as new nitrogen source for L-tryptophan fermentation by E.coli.

Authors:  Da Xu; Zhen Zhang; Ziqiang Liu; Qingyang Xu
Journal:  Bioengineered       Date:  2020-12       Impact factor: 3.269
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