Ivany Machado de Carvalho Baptista1, Frederico Canato Martinho1, Gustavo Giacomelli Nascimento2, Carlos Eduardo da Rocha Santos1, Renata Falchete do Prado3, Marcia Carneiro Valera1. 1. Institute of Science and Technology, UNESP - Univ Estadual Paulista, Av. Eng. Francisco José Longo, 777, Jd São Dimas, São José dos Campos, São Paulo, Brazil. 2. Section of Periodontology, Department of Dentistry and Oral Health, Aarhus University, Aarhus, Denmark. 3. Institute of Science and Technology, UNESP - Univ Estadual Paulista, Av. Eng. Francisco José Longo, 777, Jd São Dimas, São José dos Campos, São Paulo, Brazil. Electronic address: renatafalchete@hotmail.com.
Abstract
OBJECTIVE: To investigate the microbial diversity existing in oral cavity and respiratory tract samples (from mini-bronchoalveolar lavage (BAL), endotracheal aspirate, and orotracheal tube) of patients on mechanical ventilation by using the checkerboard DNA-DNA hybridisation. Also, the study aimed to evaluate whether the microbial profile in the oral cavity is found in respiratory tract samples, at different periods of mechanical ventilation time (12h, 48h, 96h) in attempt to identification of relationship between VAP (ventilator-associated pneumonia) and bacterial species studied. The last objective was to analyses correlation between blood cultures and VAP. DESIGN: The samples were collected from ten patients in intensive care unit with medical prescription of orotracheal intubation and mechanical ventilation. Clinical data were tabulated and blood cultures were performed according medical indication. For checkerboard samples collection, chosen sites were the dorsal side of the tongue and gingival sulcus at 12h, 48h, 96h, BAL, at 12h, endotracheal aspirate at 48 and 96h, and orotracheal tube at extubation time, when feasible. RESULTS: It was possible to identify the presence of bacterial species in mouth and in the BAL/endotracheal aspirate. The data demonstrated an increase in the quantity of bacterial associated with prolonged use of mechanical ventilation (48 and 96h). CONCLUSIONS: Bacterial species may migrate rapidly from mouth and upper airways during orotracheal intubation which contributes to the pathogenesis of VAP. There were associations between VAP and Enterococcus faecalis, Fusobacterium periodonticum, Gemella morbillorum, Neisseria mucosa, Propionibacterium acnes, Prevotella melaninogenica, Streptococcus oralis, Streptococcus sanguinis, Treponema denticola, Treponema socransckii, and Veillonella parvula.
OBJECTIVE: To investigate the microbial diversity existing in oral cavity and respiratory tract samples (from mini-bronchoalveolar lavage (BAL), endotracheal aspirate, and orotracheal tube) of patients on mechanical ventilation by using the checkerboard DNA-DNA hybridisation. Also, the study aimed to evaluate whether the microbial profile in the oral cavity is found in respiratory tract samples, at different periods of mechanical ventilation time (12h, 48h, 96h) in attempt to identification of relationship between VAP (ventilator-associated pneumonia) and bacterial species studied. The last objective was to analyses correlation between blood cultures and VAP. DESIGN: The samples were collected from ten patients in intensive care unit with medical prescription of orotracheal intubation and mechanical ventilation. Clinical data were tabulated and blood cultures were performed according medical indication. For checkerboard samples collection, chosen sites were the dorsal side of the tongue and gingival sulcus at 12h, 48h, 96h, BAL, at 12h, endotracheal aspirate at 48 and 96h, and orotracheal tube at extubation time, when feasible. RESULTS: It was possible to identify the presence of bacterial species in mouth and in the BAL/endotracheal aspirate. The data demonstrated an increase in the quantity of bacterial associated with prolonged use of mechanical ventilation (48 and 96h). CONCLUSIONS: Bacterial species may migrate rapidly from mouth and upper airways during orotracheal intubation which contributes to the pathogenesis of VAP. There were associations between VAP and Enterococcus faecalis, Fusobacterium periodonticum, Gemella morbillorum, Neisseria mucosa, Propionibacterium acnes, Prevotella melaninogenica, Streptococcus oralis, Streptococcus sanguinis, Treponema denticola, Treponema socransckii, and Veillonella parvula.
Authors: Keiko M Tarquinio; Todd Karsies; Steven L Shein; Andrew Beardsley; Robinder Khemani; Adam Schwarz; Lincoln Smith; Heidi Flori; Oliver Karam; Quy Cao; Zainab Haider; Ekaterina Smirnova; Myrna G Serrano; Gregory A Buck; Douglas F Willson Journal: Pediatr Pulmonol Date: 2021-12-02
Authors: Erika Alejandra Cifuentes; Maria A Sierra; Andrés Felipe Yepes; Ana Margarita Baldión; José Antonio Rojas; Carlos Arturo Álvarez-Moreno; Juan Manuel Anzola; María Mercedes Zambrano; Monica G Huertas Journal: Respir Res Date: 2022-06-24
Authors: Denise Battaglini; Chiara Robba; Andrea Fedele; Sebastian Trancǎ; Samir Giuseppe Sukkar; Vincenzo Di Pilato; Matteo Bassetti; Daniele Roberto Giacobbe; Antonio Vena; Nicolò Patroniti; Lorenzo Ball; Iole Brunetti; Antoni Torres Martí; Patricia Rieken Macedo Rocco; Paolo Pelosi Journal: Front Med (Lausanne) Date: 2021-06-04