| Literature DB >> 29030976 |
Ya-Fang Chen1,2, Sudhir Pandey1, Cecilia Hsuan Day3, Yu-Feng Chen4, Ai-Zhi Jiang1, Tsung-Jung Ho5, Ray-Jade Chen6, Vijaya V Padma7, Wei-Wen Kuo8, Chih-Yang Huang1,5,9,10.
Abstract
Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1α and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.Entities:
Keywords: BCL2/Adenovirus E1B Nineteen kilo Dalton Interacting Protein 3 (BNIP3); diabetic hyperglycemic; forkhead box O3 (FoxO3); hyperglycemia; hypoxia-inducible factor-1 alpha (HIF-1α); ischemia hypoxia
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Year: 2017 PMID： 29030976 DOI： 10.1002/jcp.26235
Source DB: PubMed Journal: J Cell Physiol ISSN： 0021-9541 Impact factor: 6.384