Literature DB >> 29030817

Preparation and Analysis of Saccharomyces cerevisiae Quiescent Cells.

Marla M Spain1, Sarah G Swygert1, Toshio Tsukiyama2.   

Abstract

Saccharomyces cerevisiae enter quiescence during extended growth in culture (greater than 7 days). Here, we describe a method to separate quiescent from non-quiescent cells by density gradient. We also describe approaches for DAPI staining the chromatin of quiescent cells, measuring quiescent cell viability, and extracting RNA from quiescent cells for use in genomics experiments.

Entities:  

Keywords:  DAPI; DNA; Protein; Quiescence; Quiescent cells; RNA; Saccharomyces cerevisiae; Viability

Mesh：

Substances：

Year:  2018        PMID： 29030817     DOI： 10.1007/978-1-4939-7371-2_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells.

Authors:  Sarah G Swygert; Dejun Lin; Stephanie Portillo-Ledesma; Po-Yen Lin; Dakota R Hunt; Cheng-Fu Kao; Tamar Schlick; William S Noble; Toshio Tsukiyama
Journal:  Elife       Date:  2021-11-04       Impact factor: 8.140

Review 2.  Is There a Histone Code for Cellular Quiescence?

Authors:  Kenya Bonitto; Kirthana Sarathy; Kaiser Atai; Mithun Mitra; Hilary A Coller
Journal:  Front Cell Dev Biol       Date:  2021-10-29

3.  Condensin-Dependent Chromatin Compaction Represses Transcription Globally during Quiescence.

Authors:  Sarah G Swygert; Seungsoo Kim; Xiaoying Wu; Tianhong Fu; Tsung-Han Hsieh; Oliver J Rando; Robert N Eisenman; Jay Shendure; Jeffrey N McKnight; Toshio Tsukiyama
Journal:  Mol Cell       Date:  2018-12-27       Impact factor: 17.970

4.  Chromatin structure restricts origin utilization when quiescent cells re-enter the cell cycle.

Authors:  Po-Hsuen Lee; Mary Ann Osley
Journal:  Nucleic Acids Res       Date:  2021-01-25       Impact factor: 16.971
  4 in total

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