Literature DB >> 29030811

Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining.

Ayad Eddaoudi1, Stephanie Louise Canning2, Itaru Kato3.   

Abstract

Hoechst 33342 and Pyronin Y double staining can be used to measure DNA and RNA content in live cells by flow cytometry. Quiescent cells at G0 phase have the same amount of DNA as cells at G1 phase but lower RNA levels compared to proliferating cells. Therefore, resting cells in G0 phase can be distinguished from proliferating cells in G1, S, and G2 M phases. This chapter describes a protocol for double staining of live cells with Hoechst 33342 and Pyronin Y. Combined with immunophenotyping of intact and live cells Hoechst 33342 and Pyronin Y staining is a powerful noninvasive method for the analysis and isolation of quiescent cells from any defined cell population.

Entities:  

Keywords:  Cell cycle; G0; Hoechst 33342; Live-cell sorti ng; Multiparametric flow cytometry; Pyronin Y

Mesh：

Substances：

Year:  2018        PMID： 29030811     DOI： 10.1007/978-1-4939-7371-2_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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