Yachao Zhao1, Lijun Wang2, Zhiyi Zhang1, Jiao Feng1, Huaixing Kang3, Liqun Fang1, Xiaoyuan Jiang1, Defu Zhang1,4, Zhe Zhan1, Dongsheng Zhou1, Yigang Tong1. 1. State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China. 2. Department of Laboratory Medicine, Beijing Tsinghua Chang Gung Hospital, Tsinghua University, Beijing 102218, China. 3. The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China. 4. College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China.
Abstract
AIM: To characterize a conjugative bla NDM-1-carrying plasmid pNDM-BTR from a clinical Escherichia coli isolate. MATERIALS & METHODS: The complete nucleotide sequence of pNDM-BTR was determined using next-generation sequencing technology. Comparative genomic analysis of bla NDM-carrying IncN1 plasmids, including pNDM-BTR, was performed, and the antimicrobial resistance phenotypes were determined. RESULTS: pNDM-BTR contained three accessory modules, namely IS26, a novel Tn3-family transposon Tn6360 and the dfrA14 region composed of In191, ecoRII-ecoRIImet and ΔIS1X2. The relatively small IncN1 backbones could integrate massive accessory modules, most of which were integrated at two 'hotspots'. These IncN1 plasmids contained distinct profiles of accessory modules, which included those carrying various resistance genes. CONCLUSION: This study provides a deeper insight into horizontal transfer of resistance genes among IncN1 plasmids.
AIM: To characterize a conjugative bla NDM-1-carrying plasmid pNDM-BTR from a clinical Escherichia coli isolate. MATERIALS & METHODS: The complete nucleotide sequence of pNDM-BTR was determined using next-generation sequencing technology. Comparative genomic analysis of bla NDM-carrying IncN1 plasmids, including pNDM-BTR, was performed, and the antimicrobial resistance phenotypes were determined. RESULTS: pNDM-BTR contained three accessory modules, namely IS26, a novel Tn3-family transposon Tn6360 and the dfrA14 region composed of In191, ecoRII-ecoRIImet and ΔIS1X2. The relatively small IncN1 backbones could integrate massive accessory modules, most of which were integrated at two 'hotspots'. These IncN1 plasmids contained distinct profiles of accessory modules, which included those carrying various resistance genes. CONCLUSION: This study provides a deeper insight into horizontal transfer of resistance genes among IncN1 plasmids.
Entities:
Keywords:
IncN1 plasmid; bla NDM-1; mobile elements