Draft Genome Sequence of Romboutsia maritimum sp. nov. Strain CCRI-22766T, Isolated from Coastal Estuarine Mud.

The Romboutsia maritimum sp. nov. CCRI-22766T strain was isolated from coastal estuarine mud in New Zealand. The genome assembly comprised 2,854,352 bp, with 27.1% G+C content. This is the first documentation that reports the genome sequence of R. maritimum.

GENOME ANNOUNCEMENT

Romboutsia maritimum (Romboutsia maritimum [ma.ri.ti’mum. N.L. masc. gen. n. maritimum of maritime, pertaining to the isolation source]) CCRI-22766T was isolated from a coastal estuarine mud sample in New Zealand. A comparative 16S rRNA gene sequence analysis showed that the closest cultured relative of strain CCRI-22766T was Romboutsia ilealis CRIBT (97.8% identity; GenBank accession number LN555523, positions 2388619 to 2390111) (1). The genome of Romboutsia ilealis CRIBT was also compared to that of strain CCRI-22766T for genomic relatedness assessment (accession number LN555523). The genomic average nucleotide identity obtained based on BLAST (ANIb) (2) was 77.7%. Since ANIb values around 95% corresponded to the 70% DNA-DNA hybridization cutoff value for species discrimination, strain CCRI-22766T represents a novel species of the genus Romboutsia (3).

Romboutsia maritimum is a rod-shaped, strictly anaerobic, and spore-forming bacterium. It grows on blood agar in 24 h at 35°C incubated under an anaerobic atmosphere, as previously described (4), forming 6-mm colonies. Genomic DNA was isolated using the BioSprint 15 DNA blood kit (Qiagen) automated with a KingFisher mL instrument (Thermo Fisher Scientific). Whole-genome sequencing of strain CCRI-22766T was performed on an Illumina HiSeq 2500 instrument using SBS version 4 to sequence a 126-bp paired-end library (Nextera XT [Illumina]). A total of 22,483,311 reads were assembled de novo in 95 contigs using the Ray software (version 2.3.0) (5). The total genome length is 2,854,352 bp (N50, 77,093 bp), with an average G+C content of 27.1% (6). The draft genome sequence was annotated using NCBI GenBank annotation pipeline (version 4.2) and Rapid Annotations using Subsystems Technology (RAST) annotation server (version 2.0) (7). A total of 2,867 features were identified, including 36 rRNAs (complete or partial) and 56 tRNAs. Of the 2,729 putative protein-coding sequences, 1,006 were assigned as hypothetical proteins.

Accession number(s).

This whole-genome shotgun project of R. maritimum CCRI-22766T has been deposited at DDBJ/ENA/GenBank under the accession number NOJZ00000000. The version described in this paper is version NOJZ01000000.