Literature DB >> 2902165

Techniques to measure DNA single-strand breaks in cells: a review.

G Ahnström1.   

Abstract

Alkaline sucrose sedimentation was for a number of years the standard procedure for the measurement of single-strand breaks. Some years ago a number of new techniques with improved sensitivity were introduced. The following techniques are presented and discussed: alkaline unwinding, alkaline filter elution, nucleoid sedimentation, viscoelastometry, microelectrophoresis of single cells, DNA precipitation, pulse field gel electrophoresis, fluctuation spectroscopy and nick translation.

Entities:  

Mesh:

Substances:

Year:  1988        PMID: 2902165     DOI: 10.1080/09553008814552151

Source DB:  PubMed          Journal:  Int J Radiat Biol        ISSN: 0955-3002            Impact factor:   2.694


  6 in total

1.  Diagnosis of Xeroderma Pigmentosum and Related DNA Repair-Deficient Cutaneous Diseases.

Authors:  James E Cleaver
Journal:  Curr Med Lit Dermatol       Date:  2008

2.  Single cell trapping and DNA damage analysis using microwell arrays.

Authors:  David K Wood; David M Weingeist; Sangeeta N Bhatia; Bevin P Engelward
Journal:  Proc Natl Acad Sci U S A       Date:  2010-05-13       Impact factor: 11.205

Review 3.  Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility.

Authors:  Monis Bilal Shamsi; Syed Nazar Imam; Rima Dada
Journal:  J Assist Reprod Genet       Date:  2011-09-09       Impact factor: 3.412

4.  DNA fragmentation in microorganisms assessed in situ.

Authors:  José Luis Fernández; Mónica Cartelle; Lourdes Muriel; Rebeca Santiso; María Tamayo; Vicente Goyanes; Jaime Gosálvez; Germán Bou
Journal:  Appl Environ Microbiol       Date:  2008-08-08       Impact factor: 4.792

5.  Application of alkaline unwinding assay for detection of mutagen-induced DNA strand breaks.

Authors:  M Dusinská; D Slamenová
Journal:  Cell Biol Toxicol       Date:  1992 Oct-Dec       Impact factor: 6.691

Review 6.  Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay.

Authors:  Elva I Cortés-Gutiérrez; Carmen López-Fernández; José Luis Fernández; Martha I Dávila-Rodríguez; Stephen D Johnston; Jaime Gosálvez
Journal:  Front Genet       Date:  2014-11-27       Impact factor: 4.599

  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.