Literature DB >> 29021

Isoelectric focusing studies on the neutral 5'-nucleotidase from Wistar rat hippocampus: evidence for the absence of isoenzymes.

H G Bernstein, H Luppa.   

Abstract

The pattern and some substrates characteristic of the rat brain 5'-nucleotidase were studied using the isoelectric focusing technique, which revealed that the enzyme is present in a single form in hippocampus extracts. An alkaline phosphatase, which is also able to split nucleoside monophosphates, is not active at neutral pH values. The isoelectric points were found to be 6.4 +/- 0.1 for the specific 5'-nucleotidase and 6.8 +/- 0.1 for the phosphatase.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 29021     DOI： 10.1007/BF00495995

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  11 in total

1.  Histochemistry of hepatic phosphatases of a physiologic pH; with special reference to the demonstration of bile canaliculi.

Authors:  M WACHSTEIN; E MEISEL
Journal:  Am J Clin Pathol       Date:  1957-01       Impact factor: 2.493

2.  Effect of sodium deoxycholate on 5'-nucleotidase.

Authors:  S Nakamura
Journal:  Biochim Biophys Acta       Date:  1976-03-05

3.  Purine catabolism in man: characterization of placental microsomal 5'-nucleotidase.

Authors:  I H Fox; P J Marchant
Journal:  Can J Biochem       Date:  1976-05

4.  Kinetic properties of cerebral 5'-nucleotidase.

Authors:  H Kluge; W Hartmann; V Wieczorek; W Zahlten
Journal:  J Neurochem       Date:  1972-05       Impact factor: 5.372

5.  [Acid 5'-nucleotidase in the central nervous system of the white rat].

Authors:  M Davidoff; G Galabov
Journal:  Histochemie       Date:  1971

6.  5'-nucleotidase. I. Distribution of 5'-nucleotidase in tissues of rat and mouse.

Authors:  M J Hardonk
Journal:  Histochemie       Date:  1968

7.  5'-nucleotidase. 3. Determinations of 5'-nucleotidase isoenzymes in tissues of rat and mouse.

Authors:  M J Hardonk; H G BDe Boer
Journal:  Histochemie       Date:  1968

8.  [Demonstration of thiaminpyrophosphatase (TPPase) in the hippocampus region of the wistar rat with the isofocussing method (author's transl)].

Authors:  H G Bernstein; H Luppa
Journal:  Acta Histochem       Date:  1976       Impact factor: 2.479

9.  Cytochemical investigations on the localization of 5'-nucleotidase in the rat hippocampus with special reference to synaptic regions.

Authors:  H G Bernstein; J Weiss; H Luppa
Journal:  Histochemistry       Date:  1978-04-04

10.  The specificity of 5'-nucleotidase in the brain of the mouse.

Authors:  T G Scott
Journal:  J Histochem Cytochem       Date:  1965 Nov-Dec       Impact factor: 2.479
View more
  1 in total

1.  Failure to demonstrate rat hippocampus adenylate cyclase.

Authors:  G Poeggel; H G Bernstein; H Luppa
Journal:  Histochemistry       Date:  1980
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.