Taru Aggarwal1, Ridhima Wadhwa1, Vishwajeet Rohil2, Pawan Kumar Maurya1. 1. a Centre for Medical Biotechnology, Amity Institute of Biotechnology , Amity University Uttar Pradesh , Noida , India. 2. b Department of Clinical Biochemistry , Vallabhbhai Patel Chest Institute, Delhi University , India.
Abstract
CONTENT: The increased oxidative stress in chronic obstructive pulmonary disease (COPD) patients is the result of increased inhaled oxidants, generated by various cells of the airways. OBJECTIVE: The investigation included measurements of malondiadehyde (MDA), uric acid, ascorbic acid, and matrix metalloproteinase-12 (MMP-12) in COPD patient. We also performed genetic analysis for protein-protein interaction (PPI) network. MATERIALS AND METHODS: The study was conducted on healthy subjects with normal lung function (NS, 14 subjects) and 28 patients (Global Initiative for Chronic Obstructive Lung Disease (Gold) 1 and Gold 2) with COPD. RESULTS: There was significant (p < .001) increase in MMP-12, MDA and uric acid levels as compared to healthy controls. A significant (p < .001) decline in ascorbic acid level was observed in COPD patients. The PPI was found to be 0.833 which indicated that proteins present in COPD are linked. DISCUSSION AND CONCLUSION: This study suggests oxidative stress plays an important role in COPD and the PPI provide indication that proteins present in COPD are linked.
CONTENT: The increased oxidative stress in chronic obstructive pulmonary disease (COPD) patients is the result of increased inhaled oxidants, generated by various cells of the airways. OBJECTIVE: The investigation included measurements of malondiadehyde (MDA), uric acid, ascorbic acid, and matrix metalloproteinase-12 (MMP-12) in COPDpatient. We also performed genetic analysis for protein-protein interaction (PPI) network. MATERIALS AND METHODS: The study was conducted on healthy subjects with normal lung function (NS, 14 subjects) and 28 patients (Global Initiative for Chronic Obstructive Lung Disease (Gold) 1 and Gold 2) with COPD. RESULTS: There was significant (p < .001) increase in MMP-12, MDA and uric acid levels as compared to healthy controls. A significant (p < .001) decline in ascorbic acid level was observed in COPDpatients. The PPI was found to be 0.833 which indicated that proteins present in COPD are linked. DISCUSSION AND CONCLUSION: This study suggests oxidative stress plays an important role in COPD and the PPI provide indication that proteins present in COPD are linked.
Authors: Elisabetta Zinellu; Angelo Zinellu; Alessandro G Fois; Sara S Fois; Barbara Piras; Ciriaco Carru; Pietro Pirina Journal: Oxid Med Cell Longev Date: 2020-05-14 Impact factor: 6.543
Authors: E Arellano-Orden; C Calero Acuña; V Sánchez-López; C López Ramírez; R Otero-Candelera; C Marín-Hinojosa; Jl López Campos Journal: Eur Clin Respir J Date: 2022-07-08