Mariá Andrade de Carvalho Rocha1, Laís Meireles Costa Silva1, Wellson Andrade de Oliveira1, Dayseanny de Oliveira Bezerra2, Gustavo Cardoso da Silva3, Lucilene Dos Santos Silva4, Brenda Lurian do Nascimento Medeiros5, Sílvia de Araújo França Baêta6, Maria Acelina Martins de Carvalho7, Napoleão Martins Argôlo8. 1. Fellow Master degree, Postgraduate Program in Animal Science, Universidade Federal do Piauí (UFPI), Teresina-PI, Brazil. Radiography, technical procedures, obtaining platelet-rich plasma. 2. Fellow PhD degree, Postgraduate Program in Animal Science, UFPI, Teresina-PI, Brazil. Anesthetic, technical procedures. 3. Fellow PhD degree, Northeast Network of Biotechnology, UFPI, Teresina-PI, Brazil. Computerized image analysis. 4. Fellow Postdoctoral degree, National Postdoctoral Program (PNPD/CNPq), UFPI, Teresina-PI, Brazil. Photomicrographs and histopathological examinations. 5. Graduate student, UFPI, Teresina-PI, Brazil. Technical procedures. 6. PhD, Associate Professor, Department of Clinical and Veterinary Surgery, UFPI, Teresina-PI, Brazil. Histopathological examinations, supervised all phases of the study. 7. PhD, Associate Professor, Department of Veterinary Morphology, UFPI, Teresina-PI, Brazil. Bioethical supervision. 8. PhD, Associate Professor, Department of Clinical and Veterinary Surgery, UFPI, Teresina-PI, Brazil. Design of the study, supervised all phases of the study.
Abstract
PURPOSE: To assess the efficacy of allogeneic mesenchymal stem cells and xenogenic platelet rich plasma in the treatment of bone failure of osteoporotic rabbits secondary to estrogenic deprivation and iatrogenic hypercortisolism. METHODS: Eight female rabbits underwent ovarian resection and corticoid therapy to induce clinical status of osteoporosis. Four failures were produced in the tibiae, with each failure being treated with hemostatic sponge, allogenic mesenchymal stem cells, xenogenic platelet-rich plasma and the association between both. The animals were divided into two groups, evaluated radiographically and histopathologically at 30 and 60 days post treatment. RESULTS: A radiographically confirmed consolidation of bone failures treated with allogeneic mesenchymal stem cells, associated with the histopathological image of mature and immature bone tissue, without evidence of osteopenia, was compared with the other groups, in which radiolucent failures with osteopenia and fibrosis were still present, denoting the satisfactory effect of the first treatment in detriment to the others. CONCLUSION: The treatment of bone failures of rabbits with secondary osteoporosis with allogeneic mesenchymal stem cells induced greater bone consolidation with mature and immature bone tissue production (p<0.01), when compared to the other treatments.
PURPOSE: To assess the efficacy of allogeneic mesenchymal stem cells and xenogenic platelet rich plasma in the treatment of bone failure of osteoporotic rabbits secondary to estrogenic deprivation and iatrogenic hypercortisolism. METHODS: Eight female rabbits underwent ovarian resection and corticoid therapy to induce clinical status of osteoporosis. Four failures were produced in the tibiae, with each failure being treated with hemostatic sponge, allogenic mesenchymal stem cells, xenogenic platelet-rich plasma and the association between both. The animals were divided into two groups, evaluated radiographically and histopathologically at 30 and 60 days post treatment. RESULTS: A radiographically confirmed consolidation of bone failures treated with allogeneic mesenchymal stem cells, associated with the histopathological image of mature and immature bone tissue, without evidence of osteopenia, was compared with the other groups, in which radiolucent failures with osteopenia and fibrosis were still present, denoting the satisfactory effect of the first treatment in detriment to the others. CONCLUSION: The treatment of bone failures of rabbits with secondary osteoporosis with allogeneic mesenchymal stem cells induced greater bone consolidation with mature and immature bone tissue production (p<0.01), when compared to the other treatments.