Hugo Genki Kagawa Akahane1, Ricardo Zanetti Gomes2, Katia Sabrina Paludo2, Filipe Linhares3, Luana Lopes3. 1. Graduate student, School of Medicine, Universidade Estadual de Ponta Grossa (UEPG), Brazil. Scientific and intellectual content of the study, acquisition of data, technical procedures, manuscript preparation. 2. PhD, Head, Department of Medicine, UEPG, Ponta Grossa-PR, Brazil. Scientific, intellectual, conception and design of the study; interpretation of data; critical revision. 3. Graduate student, School of Medicine, UEPG, Ponta Grossa-PR, Brazil. Technical procedures, acquisition of data.
Abstract
PURPOSE: To analyze the effects of allopurinol and of post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion. METHODS: Thirty rats were used. They were divided in 5 groups: (1) group A: abdominal aortic dissection only, (2) group B: ischemia and reperfusion, (3) group C: administered allopurinol (100mg/Kg) a few hours before procedure, (4) group D: post-conditioned and (5) group E: administered allopurinol and post-conditioned. With the exception of group A, all groups were submitted to infrarenal aortic ischemia for 2 hours, and reperfusion for 72 hours. After euthanasia, lungs were removed for histological analysis. They were graded under two scores: pulmonary injury (neutrophil infiltration, interstitial edema, vascular congestion, and destruction of lung architecture) and lymphocytic score (neutrophil infiltration, lymphoid aggregate and secondary follicle). RESULTS: On the pulmonary injury score, the degree of injury was smaller than in groups D and E, when compared to group B, p<0.05. Group C did not obtain the same result (p>0,05). On the lymphocytic score, there was no statistic difference among groups, p>0.05. CONCLUSION: Both post-conditioning and the combination of allopurinol and post-conditioning were effective in remote lung protection induced by lower-limbs I/R. When used in isolation, allopurinol showed no protective effect.
PURPOSE: To analyze the effects of allopurinol and of post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion. METHODS: Thirty rats were used. They were divided in 5 groups: (1) group A: abdominal aortic dissection only, (2) group B: ischemia and reperfusion, (3) group C: administered allopurinol (100mg/Kg) a few hours before procedure, (4) group D: post-conditioned and (5) group E: administered allopurinol and post-conditioned. With the exception of group A, all groups were submitted to infrarenal aortic ischemia for 2 hours, and reperfusion for 72 hours. After euthanasia, lungs were removed for histological analysis. They were graded under two scores: pulmonary injury (neutrophil infiltration, interstitial edema, vascular congestion, and destruction of lung architecture) and lymphocytic score (neutrophil infiltration, lymphoid aggregate and secondary follicle). RESULTS: On the pulmonary injury score, the degree of injury was smaller than in groups D and E, when compared to group B, p<0.05. Group C did not obtain the same result (p>0,05). On the lymphocytic score, there was no statistic difference among groups, p>0.05. CONCLUSION: Both post-conditioning and the combination of allopurinol and post-conditioning were effective in remote lung protection induced by lower-limbs I/R. When used in isolation, allopurinol showed no protective effect.
Authors: Mustafa Orhan; Ayça Taş Tuna; Yusuf Ünal; Mustafa Arslan; Hayrullah Yazar; Şaban Cem Sezen; Sezen Irmak Gözükara; Onur Palabıyık Journal: Turk Gogus Kalp Damar Cerrahisi Derg Date: 2021-01-13 Impact factor: 0.332
Authors: Gaizka Gutiérrez-Sánchez; Ignacio García-Alonso; Jorge Gutiérrez Sáenz de Santa María; Ana Alonso-Varona; Borja Herrero de la Parte Journal: Antioxidants (Basel) Date: 2021-05-27