Lígia Reis Moura Estevão1, Ricardo Santos Simões2, Puebla Cassini-Vieira1, Maria Cecilia Campos Canesso1, Lucíola da Silva Barcelos1, Milene Alvarenga Rachid1, Cláudio Augusto Gomes da Câmara3, Joaquim Evêncio-Neto4. 1. PhD, Department of Morphology and Animal Physiology, Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil. Acquisition, analysis and interpretation of data; manuscript writing. 2. PhD, Department of Morphology and Genetics, Universidade Federal de São Paulo (UNIFESP), Brazil. Acquisition of data, histopathological examinations, manuscript preparation, critical revision. 3. PhD, Department of Chemistry, UFRPE, Recife-PE, Brazil. Acquisition and interpretation of data, statistical analysis. 4. Full Professor, Department of Morphology and Animal Physiology, UFRPE, Recife-PE, Brazil. Conception and design of the study, manuscript preparation.
Abstract
PURPOSE: To investigated the inflammatory, angiogenic and fibrogenic activities of the Schinus terebinthifolius Raddi leaves oil (STRO) on wound healing. METHODS: The excisional wound healing model was used to evaluate the effects of STRO. The mice were divided into two groups: Control, subjected to vehicle solution (ointment lanolin/vaseline base), or STRO- treated group, administered topically once a day for 3, 7 and 14 days post-excision. We evaluated the macroscopic wound closure rate; the inflammation was evaluated by leukocytes accumulation and cytokine levels in the wounds. The accumulation of neutrophil and macrophages in the wounds were determined by assaying myeloperoxidase and N-acetyl-β-D-glucosaminidase activities. The levels of TNF-α, CXCL-1 and CCL-2 in wound were evaluated by ELISA assay. Angiogenesis and collagen fibers deposition were evaluated histologically. RESULTS: We observed that macroscopic wound closure rate was improved in wounds from STRO-group than Control-group. The wounds treated with STRO promoted a reduction in leucocyte accumulation and in pro-inflammatory cytokine. Moreover, STRO treatment increased significantly the number of blood vessels and collagen fibers deposition, as compared to control group. CONCLUSION: Topical application of STRO display anti-inflammatory and angiogenic effects, as well as improvement in collagen replacement, suggesting a putative use of this herb for the development of phytomedicines to treat inflammatory diseases, including wound healing.
PURPOSE: To investigated the inflammatory, angiogenic and fibrogenic activities of the Schinus terebinthifolius Raddi leaves oil (STRO) on wound healing. METHODS: The excisional wound healing model was used to evaluate the effects of STRO. The mice were divided into two groups: Control, subjected to vehicle solution (ointment lanolin/vaseline base), or STRO- treated group, administered topically once a day for 3, 7 and 14 days post-excision. We evaluated the macroscopic wound closure rate; the inflammation was evaluated by leukocytes accumulation and cytokine levels in the wounds. The accumulation of neutrophil and macrophages in the wounds were determined by assaying myeloperoxidase and N-acetyl-β-D-glucosaminidase activities. The levels of TNF-α, CXCL-1 and CCL-2 in wound were evaluated by ELISA assay. Angiogenesis and collagen fibers deposition were evaluated histologically. RESULTS: We observed that macroscopic wound closure rate was improved in wounds from STRO-group than Control-group. The wounds treated with STRO promoted a reduction in leucocyte accumulation and in pro-inflammatory cytokine. Moreover, STRO treatment increased significantly the number of blood vessels and collagen fibers deposition, as compared to control group. CONCLUSION: Topical application of STRO display anti-inflammatory and angiogenic effects, as well as improvement in collagen replacement, suggesting a putative use of this herb for the development of phytomedicines to treat inflammatory diseases, including wound healing.
Authors: Lígia Reis de Moura Estevão; Puebla Cassini-Vieira; Ana Greice Borba Leite; Apolônia Agnes Vilar de Carvalho Bulhões; Lucíola da Silva Barcelos; Joaquim Evêncio-Neto Journal: Bio Protoc Date: 2019-07-05
Authors: José Tarcísio de Giffoni de Carvalho; Débora da Silva Baldivia; Daniel Ferreira Leite; Laura Costa Alves de Araújo; Priscilla Pereira de Toledo Espindola; Katia Avila Antunes; Paola Santos Rocha; Kely de Picoli Souza; Edson Lucas Dos Santos Journal: Oxid Med Cell Longev Date: 2019-08-25 Impact factor: 6.543