Literature DB >> 29019409

Genome Mining and Activation of a Silent PKS/NRPS Gene Cluster Direct the Production of Totopotensamides.

Ruidong Chen1, Qingbo Zhang1, Bin Tan1, Liujuan Zheng1, Huixian Li1, Yiguang Zhu1, Changsheng Zhang1.   

Abstract

A 92 kb silent hybrid polyketide and nonribosomal peptide gene cluster in marine-derived Streptomyces pactum SCSIO 02999 was activated by genetically manipulating the regulatory genes, including the knockout of two negative regulators (totR5 and totR3) and overexpression of a positive regulator totR1, to direct the production of the known totopotensamides (TPMs) A (1) and B (3) and a novel sulfonate-containing analogue TPM C (2). Inactivation of totG led to accumulation of TPM B (3) lacking the glycosyl moiety, which indicated TotG as a dedicated glycosyltransferase in the biosynthesis of 1 and 2.

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Year:  2017        PMID： 29019409     DOI： 10.1021/acs.orglett.7b02878

Source DB:  PubMed          Journal:  Org Lett        ISSN： 1523-7052            Impact factor:   6.005


  12 in total

Review 1.  Concepts and Methods to Access Novel Antibiotics from Actinomycetes.

Authors:  Joachim J Hug; Chantal D Bader; Maja Remškar; Katarina Cirnski; Rolf Müller
Journal:  Antibiotics (Basel)       Date:  2018-05-22

2.  Genome Sequencing of Streptomyces olivaceus SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining.

Authors:  Chunyan Zhang; Wenjuan Ding; Xiangjing Qin; Jianhua Ju
Journal:  Mar Drugs       Date:  2019-10-20       Impact factor: 5.118

3.  Evolutionary Histories of Type III Polyketide Synthases in Fungi.

Authors:  Jorge Carlos Navarro-Muñoz; Jérôme Collemare
Journal:  Front Microbiol       Date:  2020-01-21       Impact factor: 5.640

4.  Eliciting the silent lucensomycin biosynthetic pathway in Streptomyces cyanogenus S136 via manipulation of the global regulatory gene adpA.

Authors:  Oleksandr Yushchuk; Iryna Ostash; Eva Mösker; Iryna Vlasiuk; Maksym Deneka; Christian Rückert; Tobias Busche; Victor Fedorenko; Jörn Kalinowski; Roderich D Süssmuth; Bohdan Ostash
Journal:  Sci Rep       Date:  2021-02-10       Impact factor: 4.379

5.  Genome Mining and Evolutionary Analysis Reveal Diverse Type III Polyketide Synthase Pathways in Cyanobacteria.

Authors:  Joachim Steen Larsen; Leanne Andrea Pearson; Brett Anthony Neilan
Journal:  Genome Biol Evol       Date:  2021-04-05       Impact factor: 3.416

Review 6.  A Review: Halogenated Compounds from Marine Actinomycetes.

Authors:  Cong Wang; Weisheng Du; Huanyun Lu; Jianzhou Lan; Kailin Liang; Shugeng Cao
Journal:  Molecules       Date:  2021-05-07       Impact factor: 4.411

Review 7.  Alkaloids in Contemporary Drug Discovery to Meet Global Disease Needs.

Authors:  Sharna-Kay Daley; Geoffrey A Cordell
Journal:  Molecules       Date:  2021-06-22       Impact factor: 4.411

Review 8.  Activation of microbial secondary metabolic pathways: Avenues and challenges.

Authors:  Bikash Baral; Amir Akhgari; Mikko Metsä-Ketelä
Journal:  Synth Syst Biotechnol       Date:  2018-09-12

9.  Streptomyces sp. VN1, a producer of diverse metabolites including non-natural furan-type anticancer compound.

Authors:  Hue Thi Nguyen; Anaya Raj Pokhrel; Chung Thanh Nguyen; Van Thuy Thi Pham; Dipesh Dhakal; Haet Nim Lim; Hye Jin Jung; Tae-Su Kim; Tokutaro Yamaguchi; Jae Kyung Sohng
Journal:  Sci Rep       Date:  2020-02-04       Impact factor: 4.379

10.  Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A.

Authors:  Taylor B Cook; Tyler B Jacobson; Maya V Venkataraman; Heike Hofstetter; Daniel Amador-Noguez; Michael G Thomas; Brian F Pfleger
Journal:  Metab Eng       Date:  2021-06-24       Impact factor: 8.829
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