Literature DB >> 2901830

Evidence of functional alpha 2-adrenergic receptors in adult-rat adipocytes by using the agonist UK 14304.

M C Rebourcet1, C Carpéné, M Lavau.   

Abstract

The aim of this study was to re-assess whether alpha 2-adrenergic receptors were present in rat adipocytes, by using UK 14304, a new and very selective alpha 2-agonist. The following observations demonstrate the presence of functional alpha 2-adrenoceptors in rat adipocytes. (1) Adipocyte lipolysis was dose-dependently inhibited by UK 14304 (maximal effect 80% at 1 microM-UK 14304, 45% at 10 microM-UK 14304, under basal or theophylline-stimulated conditions respectively). (2) UK 14304 bound specifically to purified plasma membranes, with Bmax. = 744 fmol/mg of protein and KD = 9 nM. (3) The effect of UK 14304 was suppressed by alpha 2-antagonists. (4) Adrenaline inhibited lipolysis upon beta-adrenergic blockade (propranolol). (5) The anti-lipolytic effect of UK 14304 was modulated by the age of the rats.

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Year:  1988        PMID： 2901830      PMCID： PMC1149202          DOI： 10.1042/bj2520679

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  22 in total

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2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

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Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Effects of antilipolytic agents and alpha-adrenergic antagonists on cyclic AMP metabolism in hamster white adipocytes.

Authors:  K J Hittelman; R W Butcher
Journal:  Biochim Biophys Acta       Date:  1973-09-25

4.  Adrenergic receptors for metabolic responses in adipose tissue.

Authors:  J Himms-Hagen
Journal:  Fed Proc       Date:  1970 Jul-Aug

5.  Catecholamines and metabolism of human adipose tissue. II. Effect of isoprophylnoradrenaline and adrenergic blocking agents on lipolysis in human omental adipose tissue in vitro.

Authors:  J Ostman; S Efendić
Journal:  Acta Med Scand       Date:  1970-06

6.  RX781094 a potent and selective alpha 2-adrenergic antagonist. Effects in adipocytes and hepatocytes.

Authors:  C K Pushpendran; J A García-Sáinz
Journal:  Eur J Pharmacol       Date:  1984-04-06       Impact factor: 4.432

7.  Adrenergic regulation of lipolysis in abdominal adipocytes of obese subjects during caloric restriction: reversal of catecholamine action caused by relief of endogenous inhibition.

Authors:  H Kather; E Wieland; B Fischer; A Wirth; G Schlierf
Journal:  Eur J Clin Invest       Date:  1985-02       Impact factor: 4.686

Review 8.  Heterogeneity of alpha-2 adrenergic receptors.

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Journal:  Pharmacol Biochem Behav       Date:  1985-05       Impact factor: 3.533

9.  Lack of functional antilipolytic alpha 2-adrenoceptor in rat fat cell:comparison with hamster adipocyte.

Authors:  C Carpene; M Berlan; M Lafontan
Journal:  Comp Biochem Physiol C       Date:  1983

Review 10.  Idazoxan: a novel pharmacological tool for the study of alpha 2-adrenoceptors.

Authors:  H Dabiré
Journal:  J Pharmacol       Date:  1986 Apr-Jun
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  2 in total

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