Analysis of structural and numerical chromosomal anomalies at the first, second, and third mitosis after irradiation of one-cell mouse embryos with X-rays or neutrons.

One-cell mouse embryos were irradiated with X-rays or neutrons. Analysis of the first, second, and third postradiation mitoses revealed that the yields of structural aberrations increased linearly after exposure to both radiation qualities. For X-rays the aberration frequency decreased from the first to the third mitosis, whereas after neutrons it decreased from the first to the second mitosis but then increased in the third mitosis. RBEs of 4.7, 4.8, and 7.4 were calculated for the corresponding mitoses. It was clearly demonstrated that new aberrations were produced after the first postradiation mitosis and expressed during the second and third mitosis. Chromosome loss also increased with increasing radiation dose at the second mitosis. An RBE of 2 was calculated for this effect. Comparing the presented data with previous investigations on embryonic and fetal death after prenatal irradiation, it was concluded that the high radiosensitivity of the one-cell embryo is due to the induction of structural as well as of numerical chromosome aberrations.