| Literature DB >> 28986889 |
Vijayalakshmi Rajendran1, Mayur Vilas Jain2,3.
Abstract
Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.Entities:
Keywords: Agarose; Cancer stem cell; Clonogenic assay; Colony growth; Crystal violet
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Year: 2018 PMID： 28986889 DOI： 10.1007/978-1-4939-7401-6_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745