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Literature DB >> 28986806

Production, Purification, and Titration of First-Generation Adenovirus Vectors.

Abstract

Vectors based on human adenovirus are highly efficient tools for transient genetic modifications of cells or tissues in vitro and in vivo. They can be utilized for gene addition strategies, knockdown strategies and as transfer vectors for designer nucleases and CRISPR/Cas. They are characterized by high genomic stability and can be produced to high titers. This chapter describes the method how to produce, purify and titrate adenovirus vectors based on human adenovirus type 5.

Entities: Species

Keywords: Adenovirus vector; First-generation vector; Transient genetic modification

Mesh：

Year: 2017 PMID： 28986806 DOI： 10.1007/978-1-4939-7231-9_28

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

Keyword Cloud
Cited

8 in total

1. Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting.

Authors: Franziska Jönsson; Claudia Hagedorn; Florian Kreppel
Journal: J Vis Exp Date: 2018-10-26 Impact factor: 1.355

2. Transfection of adenovirus-mediated mircoRNA-126 gene into infant hemangioma endothelial cells in vitro.

Authors: Zhe Cao; Yong-Qiang Guo; Sheng-Jian Tang; Shen-Xing Tan; Chang-Ying Niu; Chun-Lei Miao; Wei Zhang; Yu-Li Li; Long Zhang; Jing-Han Wang; Xiao-Qin Liang
Journal: Int J Clin Exp Pathol Date: 2018-03-01

3. Overexpressed ski efficiently promotes neurorestoration, increases neuronal regeneration, and reduces astrogliosis after traumatic brain injury.

Authors: Yu Zhai; Shi-Yang Ye; Qiu-Shi Wang; Ren-Ping Xiong; Sheng-Yu Fu; Hao Du; Ya-Wei Xu; Yan Peng; Zhi-Zhong Huang; Nan Yang; Yan Zhao; Ya-Lei Ning; Ping Li; Yuan-Guo Zhou
Journal: Gene Ther Date: 2022-02-08 Impact factor: 5.250

Production, Purification, and Titration of First-Generation Adenovirus Vectors.

1. Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting.

2. Transfection of adenovirus-mediated mircoRNA-126 gene into infant hemangioma endothelial cells in vitro.

3. Overexpressed ski efficiently promotes neurorestoration, increases neuronal regeneration, and reduces astrogliosis after traumatic brain injury.

4. Recording of Diurnal Gene Expression in Peripheral Organs of Mice Using the RT-Biolumicorder.

Review 5. High-Capacity Adenoviral Vectors: Expanding the Scope of Gene Therapy.

6. High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75.

7. Ski promotes proliferation and inhibits apoptosis in fibroblasts under high-glucose conditions via the FoxO1 pathway.

8. An Adenoviral Vector as a Versatile Tool for Delivery and Expression of miRNAs.