Mohsin Khurshid1,2, Muhammad Hidayat Rasool1, Usman Ali Ashfaq3, Bilal Aslam1, Muhammad Waseem1. 1. Department of Microbiology, Government College University, Faisalabad, Pakistan. 2. College of Allied Health Professionals, Directorate of Medical Sciences, Government College University, Faisalabad, Pakistan. 3. Department of Bioinformatics & Biotechnology, Government College University, Faisalabad, Pakistan.
Abstract
AIM: The ISAba1 insertion sequence supports the overexpression of various oxacillinases genes by providing promoter and therefore, confers carbapenem resistance. The study aimed to determine the clonal diversity, genetic attributes of carbapenem resistance and the role of ISAba1 in the expression of oxacillinases genes among Acinetobacter baumannii clinical isolates. MATERIALS & METHODS: PCR was performed to amplify various antimicrobial resistance determinants and ISAba1 insertion elements in 137 A. baumannii clinical isolates. RESULTS: The bla OXA51 and bla OXA23-like genes and ISAba1 upstream to these genes were found in almost all multidrug-resistant isolates belonging to multiple clones. CONCLUSION: This is the first comprehensive study from Pakistan that highlighted the emergence of ISAba1 harboring carbapenems resistant A. baumannii stains in tertiary care hospitals.
AIM: The ISAba1 insertion sequence supports the overexpression of various oxacillinases genes by providing promoter and therefore, confers carbapenem resistance. The study aimed to determine the clonal diversity, genetic attributes of carbapenem resistance and the role of ISAba1 in the expression of oxacillinases genes among Acinetobacter baumannii clinical isolates. MATERIALS & METHODS: PCR was performed to amplify various antimicrobial resistance determinants and ISAba1 insertion elements in 137 A. baumannii clinical isolates. RESULTS: The bla OXA51 and bla OXA23-like genes and ISAba1 upstream to these genes were found in almost all multidrug-resistant isolates belonging to multiple clones. CONCLUSION: This is the first comprehensive study from Pakistan that highlighted the emergence of ISAba1 harboring carbapenems resistant A. baumannii stains in tertiary care hospitals.
Authors: Mohsin Khurshid; Muhammad Hidayat Rasool; Usman Ali Ashfaq; Bilal Aslam; Muhammad Waseem; Muhammad Akhtar Ali; Ahmad Almatroudi; Farhan Rasheed; Muhammad Saeed; Qinglan Guo; Minggui Wang Journal: Infect Drug Resist Date: 2020-08-18 Impact factor: 4.003
Authors: Sana Ilyas; Muhammad Hidayat Rasool; Muhammad Javed Arshed; Muhammad Usman Qamar; Bilal Aslam; Ahmad Almatroudi; Mohsin Khurshid Journal: Infect Drug Resist Date: 2021-03-02 Impact factor: 4.003