| Literature DB >> 2897992 |
P J Armati1, J D Pollard, D Van Reyk, L Van der Lubbe.
Abstract
Immunoelectron microscopy is an important tool used to determine the precise location of immune complexes. Standard concentrations of glutaraldehyde destroy these complexes. This paper describes a method in which the period of glutaraldehyde fixation is shortened by concomitant microwave treatment. Using 1.25% glutaraldehyde and microwave fixation ideal preservation and demonstration of MHC class I antigen on Schwann cells was obtained by the peroxidase method.Entities:
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Year: 1988 PMID： 2897992 DOI： 10.1016/0022-1759(88)90113-5
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303