Amanda Silveira Denadai1,2, Ricardo Dutra Aydos1, Iandara Schettert Silva1, Larissa Olmedo3, Bruno Mendonça de Senna Cardoso4, Baldomero Antonio Kato da Silva2,5, Paulo de Tarso Camillo de Carvalho6. 1. Federal University of Mato Grosso do Sul (UFMS), Post Graduate Program in Health and Development of Midwest Region, Campo Grande/MS, Brazil. 2. Federal University of Piauí (UFPI), Department of Health Sciences, Parnaíba/PI, Brazil. 3. Federal University of Mato Grosso do Sul (UFMS), Graduate Course of Physiotherapy, Campo Grande/MS, Brazil. 4. University Anhanguera-UNIDERP, Graduate Course of Veterinary Medicine, Campo Grande/MS, Brazil. 5. Federal University of Piauí (UFPI), Postgraduate Program in Biomedical Sciences, Parnaíba/PI, Brazil. 6. University Nove de Julho (UNINOVE), Postgraduate Program in Rehabilitation Sciences and Postgraduate Program in Biophotonics, São Paulo/SP, Brazil.
Abstract
BACKGROUND: Laser therapy influences oxidative stress parameters such as the activity of antioxidant enzymes and the production of reactive oxygen species. OBJECTIVE: To analyze the effects of low-level laser therapy on oxidative stress in diabetics rats with skin wounds. METHODS: Thirty-six animals were divided into 4 groups: NDNI: non-diabetic rats with cutaneous wounds that not received laser therapy; NDI: non-diabetic rats with cutaneous wounds that received laser therapy; DNI: diabetic rats with skin wounds who did not undergo laser therapy; DI: rats with diabetes insipidus and cutaneous wounds and received laser therapy. The animals were treated with LLLT (660 nm, 100 mW, 6 J/cm, spot size 0.028 cm). On the day of killing the animals, tissue-wrapped cutaneous wounds were collected and immediately frozen, centrifuged, and stored to analyze malondialdehyde (MDA) levels. RESULTS: Significant difference was observed within the groups of MDA levels (ANOVA, p = 0.0001). Tukey's post-hoc test showed significantly lower values of MDA in irradiated tissues, both in diabetic and non-diabetic rats. ANOVA of the diabetic group revealed a significant difference (p < 0.01) when all groups, except NDI and DI, were compared. CONCLUSIONS: LLLT was effective in decreasing MDA levels in acute surgical wounds in diabetic rats.
BACKGROUND: Laser therapy influences oxidative stress parameters such as the activity of antioxidant enzymes and the production of reactive oxygen species. OBJECTIVE: To analyze the effects of low-level laser therapy on oxidative stress in diabeticsrats with skin wounds. METHODS: Thirty-six animals were divided into 4 groups: NDNI: non-diabeticrats with cutaneous wounds that not received laser therapy; NDI: non-diabeticrats with cutaneous wounds that received laser therapy; DNI: diabeticrats with skin wounds who did not undergo laser therapy; DI: rats with diabetes insipidus and cutaneous wounds and received laser therapy. The animals were treated with LLLT (660 nm, 100 mW, 6 J/cm, spot size 0.028 cm). On the day of killing the animals, tissue-wrapped cutaneous wounds were collected and immediately frozen, centrifuged, and stored to analyze malondialdehyde (MDA) levels. RESULTS: Significant difference was observed within the groups of MDA levels (ANOVA, p = 0.0001). Tukey's post-hoc test showed significantly lower values of MDA in irradiated tissues, both in diabetic and non-diabeticrats. ANOVA of the diabetic group revealed a significant difference (p < 0.01) when all groups, except NDI and DI, were compared. CONCLUSIONS: LLLT was effective in decreasing MDA levels in acute surgical wounds in diabeticrats.
Authors: Mariana de Souza Costa; Ramon Handerson Gomes Teles; Yago Medeiros Dutra; José Carlos Rapozo Mazulo Neto; Tarcisio Vieira de Brito; Francisca Francisete de Sousa Nunes Queiroz; Donária Bezerra Nobre do Vale; Luan Kelves Miranda de Souza; Irismara Sousa Silva; André Luiz Dos Reis Barbosa; Jand-Venes Rolim Medeiros; Nivaldo Antonio Parizotto; Marcelo de Carvalho Filgueiras Journal: Lasers Med Sci Date: 2018-06-28 Impact factor: 3.161
Authors: R K Mathur; Khageswar Sahu; Siddharth Saraf; Pooja Patheja; Fareed Khan; P K Gupta Journal: Lasers Med Sci Date: 2016-11-29 Impact factor: 3.161