Sabrina De Carolis1,2, Alice Pellegrini3, Donatella Santini4, Claudio Ceccarelli1, Antonio De Leo1, Federica Alessandrini1, Chiara Arienti5, Sara Pignatta5, Anna Tesei5, Vilma Mantovani2, Claudio Zamagni6, Mario Taffurelli3,7, Pasquale Sansone8,9, Massimiliano Bonafé1,2, Monica Cricca1. 1. Department of Experimental, Diagnostic & Specialty Medicine, Alma Mater Studiorum, University of Bologna, 40138, Bologna, Italy. 2. Center of Applied Biomedical Research (CRBA), S. Orsola-Malpighi Hospital, 40138, Bologna, Italy. 3. Department of Women, Children & Urological Diseases, S. Orsola-Malpighi Hospital, 40138, Bologna, Italy. 4. Operative Unit of Pathology, S. Orsola Malpighi Hospital, 40138, Bologna, Italy. 5. Drug Discovery Unit & Radiobiology, Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST), IRCCS, 47014, Meldola, Italy. 6. Medical Oncology Unit, S. Orsola-Malpighi Hospital, 40138, Bologna, Italy. 7. Department of Medical & Surgical Sciences, Alma Mater Studiorum, University of Bologna, 40138, Bologna, Italy. 8. Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, 10021 NY, USA. 9. Department of Pediatrics, Cell & Developmental Biology, Children's Cancer & Blood Foundation Laboratories, Weill Cornell Medical College, New York, 10021 NY, USA.
Abstract
AIM: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens. MATERIALS & METHODS: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types. RESULTS: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas. We also show the presence of HPV DNA in the serum-derived extracellular vesicles. CONCLUSION: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology.
AIM: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens. MATERIALS & METHODS: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types. RESULTS: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas. We also show the presence of HPV DNA in the serum-derived extracellular vesicles. CONCLUSION: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology.
Authors: Kai Dun Tang; Lilian Menezes; Kurt Baeten; Laurence J Walsh; Bernard C S Whitfield; Martin D Batstone; Liz Kenny; Ian H Frazer; Gert C Scheper; Chamindie Punyadeera Journal: Biomolecules Date: 2020-02-03
Authors: Mohammad Al Hamad; Ismail Matalka; Mazhar Salim Al Zoubi; Ivana Armogida; Rawan Khasawneh; Maysa Al-Husaini; Maher Sughayer; Saied Jaradat; Amjad D Al-Nasser; Chiara Maria Mazzanti Journal: Breast Cancer (Auckl) Date: 2020-11-24
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