J Boyer1, M-C Saint-Paul1, B Dadone1, S Patouraux1, M-H Vivinus2, D Ouvrier3, J-F Michiels1, T Piche3, M K Tulic4,5. 1. Laboratoire d'anatomopathologie (LCAP) CHU de Nice Hôpital Pasteur, Nice, France. 2. Laboratoire d'Immunologie CHU de Nice Hôpital de l'Archet 1, Nice, France. 3. Service de Gastroentérologie, CHU de Nice, Hôpital de l'Archet 2, Nice, France. 4. Centre Méditerranéen de Médecine Moléculaire (C3M Team 12), INSERM U1065, Nice, France. 5. International Inflammation Network (in-FLAME) of the World Universities Network.
Abstract
BACKGROUND: Currently, there are no histological criteria to diagnose irritable bowel syndrome (IBS). Our aims were (i) to examine the distribution of inflammatory cells in the colon of healthy and IBS subjects and (ii) to find histological diagnosis criteria for IBS. METHODS: Colonic biopsies were taken from four distinct regions of the colon from 20 controls (HC) and 11 patients with IBS (4 with constipation (IBS-C) and 7 with diarrhea (IBS-D) and embedded in paraffin. Macrophages, mast cells, eosinophils, and T lymphocytes were immunostained and positive cells counted. KEY RESULTS: In both HC and IBS patients, global cellularity decreased from the cecum to the rectum (P < .01) which is attributed to reduced number of macrophages (P < .05) and eosinophils (P < .001) but not T cells. Mast cells were reduced in IBS (P < .05) but not in HC, particularly in IBS-D (P < .05). Results showed higher number of macrophages in the left colon of IBS subjects than HC (P < .05). CONCLUSION & INFERENCES: Here we report a decreasing gradient of immune cells from the cecum to the rectum of the human colon. Although global cellularity cannot be used to distinguish between IBS and HC, closer analysis of macrophages and mast cells may be useful markers to confirm IBS histologically and to differentiate between IBS-C and IBS-D when clinical presentation alternates between constipation and diarrhoea. This pilot study remains to be confirmed with greater number of patients.
BACKGROUND: Currently, there are no histological criteria to diagnose irritable bowel syndrome (IBS). Our aims were (i) to examine the distribution of inflammatory cells in the colon of healthy and IBS subjects and (ii) to find histological diagnosis criteria for IBS. METHODS: Colonic biopsies were taken from four distinct regions of the colon from 20 controls (HC) and 11 patients with IBS (4 with constipation (IBS-C) and 7 with diarrhea (IBS-D) and embedded in paraffin. Macrophages, mast cells, eosinophils, and T lymphocytes were immunostained and positive cells counted. KEY RESULTS: In both HC and IBSpatients, global cellularity decreased from the cecum to the rectum (P < .01) which is attributed to reduced number of macrophages (P < .05) and eosinophils (P < .001) but not T cells. Mast cells were reduced in IBS (P < .05) but not in HC, particularly in IBS-D (P < .05). Results showed higher number of macrophages in the left colon of IBS subjects than HC (P < .05). CONCLUSION & INFERENCES: Here we report a decreasing gradient of immune cells from the cecum to the rectum of the human colon. Although global cellularity cannot be used to distinguish between IBS and HC, closer analysis of macrophages and mast cells may be useful markers to confirm IBS histologically and to differentiate between IBS-C and IBS-D when clinical presentation alternates between constipation and diarrhoea. This pilot study remains to be confirmed with greater number of patients.
Authors: Eloísa Salvo-Romero; Cristina Martínez; Beatriz Lobo; Bruno K Rodiño-Janeiro; Marc Pigrau; Alejandro D Sánchez-Chardi; Ana M González-Castro; Marina Fortea; Cristina Pardo-Camacho; Adoración Nieto; Elba Expósito; Danila Guagnozzi; Amanda Rodríguez-Urrutia; Inés de Torres; Ricard Farré; Fernando Azpiroz; Carmen Alonso-Cotoner; Javier Santos; María Vicario Journal: Sci Rep Date: 2020-11-26 Impact factor: 4.379