Literature DB >> 2897473

Crystallization and preliminary diffraction data for adenosylcobalamin-dependent methylmalonyl-CoA mutase from Propionibacterium shermanii.

N Marsh1, P F Leadlay, P R Evans.   

Abstract

Pink crystals of methylmalonyl-CoA mutase from Propionibacterium shermanii, a coenzyme B12 (5'-deoxyadenosylcobalamin)-dependent enzyme, have been obtained by the hanging-drop method in two different forms. One form lies in the space group P21, with unit cell dimensions a = 122 A, b = 160 A and c = 90 A, with beta = 104 degrees (1 A = 0.1 nm). There are two alpha beta dimers in the asymmetric unit. The crystals diffract to 3.2 A resolution and are suitable for high resolution X-ray diffraction studies.

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Year:  1988        PMID： 2897473     DOI： 10.1016/0022-2836(88)90252-5

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  8 in total

1.  Adenosylcobalamin-dependent methylmalonyl-CoA mutase from Propionibacterium shermanii. Active holoenzyme produced from Escherichia coli.

Authors:  N McKie; N H Keep; M L Patchett; P F Leadlay
Journal:  Biochem J       Date:  1990-07-15       Impact factor: 3.857

2.  Subunit interactions in Propionibacterium shermanii methylmalonyl-CoA mutase studied by analytical ultracentrifugation.

Authors:  E N Marsh; S E Harding; P F Leadlay
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857

3.  Methylmalonyl-CoA mutase from Propionibacterium shermanii. Evidence for the presence of two masked cysteine residues.

Authors:  E N Marsh; P F Leadlay
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857

4.  Cloning and structural characterization of the genes coding for adenosylcobalamin-dependent methylmalonyl-CoA mutase from Propionibacterium shermanii.

Authors:  E N Marsh; N McKie; N K Davis; P F Leadlay
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857

5.  Molecular basis for dysfunction of some mutant forms of methylmalonyl-CoA mutase: deductions from the structure of methionine synthase.

Authors:  C L Drennan; R G Matthews; D S Rosenblatt; F D Ledley; W A Fenton; M L Ludwig
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-28       Impact factor: 11.205

6.  Cloning and expression of a mutant methylmalonyl coenzyme A mutase with altered cobalamin affinity that causes mut- methylmalonic aciduria.

Authors:  A M Crane; R Jansen; E R Andrews; F D Ledley
Journal:  J Clin Invest       Date:  1992-02       Impact factor: 14.808

7.  Primary structure and activity of mouse methylmalonyl-CoA mutase.

Authors:  M F Wilkemeyer; A M Crane; F D Ledley
Journal:  Biochem J       Date:  1990-10-15       Impact factor: 3.857

8.  The synthetic substrate succinyl(carbadethia)-CoA generates cob(II)alamin on adenosylcobalamin-dependent methylmalonyl-CoA mutase.

Authors:  N H Keep; G A Smith; M C Evans; G P Diakun; P F Leadlay
Journal:  Biochem J       Date:  1993-10-15       Impact factor: 3.857
  8 in total

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