Literature DB >> 28973886

Temporal calcium profiling of specific circadian neurons in freely moving flies.

Fang Guo1,2,3, Xiao Chen2,3, Michael Rosbash4,2,3.   

Abstract

There are no general methods for reliably assessing the firing properties or even calcium profiles of specific neurons in freely moving flies. To this end, we adapted a GFP-based calcium reporter to luciferase that was expressed in small subsets of circadian neurons. This Tric-LUC reporter allowed a direct comparison of luciferase activity with locomotor activity, which was assayed in the same flies with video recording. The LUC profile from activity-promoting E cells paralleled evening locomotor activity, and the LUC profile from sleep-promoting glutamatergic DN1s (gDN1s) paralleled daytime sleep. Similar profiles were generated by novel reporters recently identified based on transcription factor activation. As E cell and gDN1 activity is necessary and sufficient for normal evening locomotor activity and daytime sleep profiles, respectively, we suggest that their luciferase profiles reflect their neuronal calcium and in some cases firing profiles in wake-behaving flies.

Entities:  

Keywords:  Drosophila; calcium; circadian; optogenetics; sleep

Mesh：

Substances：

Year:  2017        PMID： 28973886      PMCID： PMC5642695          DOI： 10.1073/pnas.1706608114

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  39 in total

1.  A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability.

Authors:  Matthieu Flourakis; Elzbieta Kula-Eversole; Alan L Hutchison; Tae Hee Han; Kimberly Aranda; Devon L Moose; Kevin P White; Aaron R Dinner; Bridget C Lear; Dejian Ren; Casey O Diekman; Indira M Raman; Ravi Allada
Journal:  Cell       Date:  2015-08-13       Impact factor: 41.582

2.  Synchronous Drosophila circadian pacemakers display nonsynchronous Ca²⁺ rhythms in vivo.

Authors:  Xitong Liang; Timothy E Holy; Paul H Taghert
Journal:  Science       Date:  2016-02-26       Impact factor: 47.728

3.  Optogenetic inhibition of behavior with anion channelrhodopsins.

Authors:  Farhan Mohammad; James C Stewart; Stanislav Ott; Katarina Chlebikova; Jia Yi Chua; Tong-Wey Koh; Joses Ho; Adam Claridge-Chang
Journal:  Nat Methods       Date:  2017-01-23       Impact factor: 28.547

4.  A pdf neuropeptide gene mutation and ablation of PDF neurons each cause severe abnormalities of behavioral circadian rhythms in Drosophila.

Authors:  S C Renn; J H Park; M Rosbash; J C Hall; P H Taghert
Journal:  Cell       Date:  1999-12-23       Impact factor: 41.582

5.  DN1(p) circadian neurons coordinate acute light and PDF inputs to produce robust daily behavior in Drosophila.

Authors:  Luoying Zhang; Brian Y Chung; Bridget C Lear; Valerie L Kilman; Yixiao Liu; Guruswamy Mahesh; Rose-Anne Meissner; Paul E Hardin; Ravi Allada
Journal:  Curr Biol       Date:  2010-04-01       Impact factor: 10.834

Review 6.  Studying circadian rhythms in Drosophila melanogaster.

Authors:  Ozgur Tataroglu; Patrick Emery
Journal:  Methods       Date:  2014-01-09       Impact factor: 3.608

7.  The Drosophila circadian clock is a variably coupled network of multiple peptidergic units.

Authors:  Z Yao; O T Shafer
Journal:  Science       Date:  2014-03-28       Impact factor: 47.728

8.  Differential control of morning and evening components in the activity rhythm of Drosophila melanogaster--sex-specific differences suggest a different quality of activity.

Authors:  C Helfrich-Förster
Journal:  J Biol Rhythms       Date:  2000-04       Impact factor: 3.182

9.  Light evokes rapid circadian network oscillator desynchrony followed by gradual phase retuning of synchrony.

Authors:  Logan Roberts; Tanya L Leise; Takako Noguchi; Alexis M Galschiodt; Jerry H Houl; David K Welsh; Todd C Holmes
Journal:  Curr Biol       Date:  2015-03-05       Impact factor: 10.834

10.  In vivo circadian oscillation of dCREB2 and NF-κB activity in the Drosophila nervous system.

Authors:  Anne K Tanenhaus; Jiabin Zhang; Jerry C P Yin
Journal:  PLoS One       Date:  2012-10-15       Impact factor: 3.240
View more
  28 in total

1.  Activity-dependent visualization and control of neural circuits for courtship behavior in the fly Drosophila melanogaster.

Authors:  Seika Takayanagi-Kiya; Taketoshi Kiya
Journal:  Proc Natl Acad Sci U S A       Date:  2019-03-05       Impact factor: 11.205

Review 2.  Recent Advances in the Genetic Dissection of Neural Circuits in Drosophila.

Authors:  Chao Guo; Yufeng Pan; Zhefeng Gong
Journal:  Neurosci Bull       Date:  2019-05-22       Impact factor: 5.203

3.  GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior.

Authors:  Takashi Maejima; Yusuke Tsuno; Shota Miyazaki; Yousuke Tsuneoka; Emi Hasegawa; Md Tarikul Islam; Ryosuke Enoki; Takahiro J Nakamura; Michihiro Mieda
Journal:  Proc Natl Acad Sci U S A       Date:  2021-02-09       Impact factor: 11.205

4.  Neuronal Activity in Non-LNv Clock Cells Is Required to Produce Free-Running Rest:Activity Rhythms in Drosophila.

Authors:  Nicholas Bulthuis; Katrina R Spontak; Benjamin Kleeman; Daniel J Cavanaugh
Journal:  J Biol Rhythms       Date:  2019-04-17       Impact factor: 3.182

5.  A Distinct Visual Pathway Mediates High-Intensity Light Adaptation of the Circadian Clock in Drosophila.

Authors:  Matthias Schlichting; Pamela Menegazzi; Michael Rosbash; Charlotte Helfrich-Förster
Journal:  J Neurosci       Date:  2019-01-03       Impact factor: 6.167

6.  Allatostatin-C/AstC-R2 Is a Novel Pathway to Modulate the Circadian Activity Pattern in Drosophila.

Authors:  Madelen M Díaz; Matthias Schlichting; Katharine C Abruzzi; Xi Long; Michael Rosbash
Journal:  Curr Biol       Date:  2018-12-13       Impact factor: 10.834

Review 7.  The role of nuclear Ca2+ in maintaining neuronal homeostasis and brain health.

Authors:  Pawel Mozolewski; Maciej Jeziorek; Christoph M Schuster; Hilmar Bading; Bess Frost; Radek Dobrowolski
Journal:  J Cell Sci       Date:  2021-04-22       Impact factor: 5.285

8.  Repetitive mild head trauma induces activity mediated lifelong brain deficits in a novel Drosophila model.

Authors:  Joseph A Behnke; Changtian Ye; Aayush Setty; Kenneth H Moberg; James Q Zheng
Journal:  Sci Rep       Date:  2021-05-06       Impact factor: 4.379

9.  NonA and CPX Link the Circadian Clockwork to Locomotor Activity in Drosophila.

Authors:  Weifei Luo; Fang Guo; Aoife McMahon; Shalise Couvertier; Hua Jin; Madelen Diaz; Allegra Fieldsend; Eranthie Weerapana; Michael Rosbash
Journal:  Neuron       Date:  2018-07-26       Impact factor: 17.173

10.  Weekend Light Shifts Evoke Persistent Drosophila Circadian Neural Network Desynchrony.

Authors:  Ceazar Nave; Logan Roberts; Patrick Hwu; Jerson D Estrella; Thanh C Vo; Thanh H Nguyen; Tony Thai Bui; Daniel J Rindner; Nicholas Pervolarakis; Paul J Shaw; Tanya L Leise; Todd C Holmes
Journal:  J Neurosci       Date:  2021-04-30       Impact factor: 6.167
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.