CRISPR-RT: a web application for designing CRISPR-C2c2 crRNA with improved target specificity.

Summary: CRISPR-Cas systems have been successfully applied in genome editing. Recently, the CRISPR-C2c2 system has been reported as a tool for RNA editing. Here we describe CRISPR-RT (CRISPR RNA-Targeting), the first web application to help biologists design crRNAs with improved target specificity for the CRISPR-C2c2 system. CRISPR-RT allows users to set up a wide range of parameters, making it highly flexible for current and future research in CRISPR-based RNA editing. CRISPR-RT covers major model organisms and can be easily extended to cover other species. CRISPR-RT will empower researchers in RNA editing. Availability and implementation: Freely available at http://bioinfolab.miamioh.edu/CRISPR-RT. Contact: liangc@miamioh.edu. Supplementary information: Supplementary data are available at Bioinformatics online.