Qiman Ma1, Liangyuan Li2, Yan Tang3, Qiang Fu4, Sheng Liu5, Shengwei Hu6, Jun Qiao5, Chuangfu Chen7, Wei Ni8. 1. College of Medicine, Shihezi University, Shihezi, Xinjiang 832003, China. 2. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China; State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, Xinjiang 832000, China. 3. Animal Science and Technology Branch, Xinjiang Agricultural Vocational Technical College, Changji, Xinjiang 831100, China. 4. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China. 5. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China. 6. College of Life Technology, Shihezi University, Shihezi, Xinjiang 832003, China. Electronic address: 28021883@qq.com. 7. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China. Electronic address: chuangfu666@yeah.net. 8. College of Life Technology, Shihezi University, Shihezi, Xinjiang 832003, China. Electronic address: niweiwonderful@sina.com.
Abstract
BACKGROUND: Bovine viral diarrhea virus (BVDV) infection is a dynamic and complex process that leads to significant economic losses in the dairy and cattle industries. However, our understanding of the protective and pathological mechanism underlying host infection is limited. METHODS: To determine whether BVDV regulates specific activities of the host cell, the expression of long non-coding RNA (lncRNA) during BVDV NADL infection was studied by deep sequencing. RESULTS: A total of 1236 lncRNA transcripts and 3261 mRNA transcripts were differentially regulated at 2h, 6h, and 18h post-infection. The lncRNAs shared same characteristics with other mammals in terms of exon length, number, expression level, and conservation. The Gene Ontology (GO) enrichment and KEGG pathway analyses showed that lncRNAs regulate immune reaction during BVDV infection. Thirteen differentially expressed genes in 18 hpi were selected and independently validated by reverse-transcription qPCR. CONCLUSIONS: The present study is the first to provide insights into the biological connection of lncRNAs and BVDV, which can be further explored for the development of antiviral prevention strategies and in understanding persistent infection between viral and host components.
BACKGROUND: Bovine viral diarrhea virus (BVDV) infection is a dynamic and complex process that leads to significant economic losses in the dairy and cattle industries. However, our understanding of the protective and pathological mechanism underlying host infection is limited. METHODS: To determine whether BVDV regulates specific activities of the host cell, the expression of long non-coding RNA (lncRNA) during BVDV NADL infection was studied by deep sequencing. RESULTS: A total of 1236 lncRNA transcripts and 3261 mRNA transcripts were differentially regulated at 2h, 6h, and 18h post-infection. The lncRNAs shared same characteristics with other mammals in terms of exon length, number, expression level, and conservation. The Gene Ontology (GO) enrichment and KEGG pathway analyses showed that lncRNAs regulate immune reaction during BVDV infection. Thirteen differentially expressed genes in 18 hpi were selected and independently validated by reverse-transcription qPCR. CONCLUSIONS: The present study is the first to provide insights into the biological connection of lncRNAs and BVDV, which can be further explored for the development of antiviral prevention strategies and in understanding persistent infection between viral and host components.
Authors: Yan Xu; Jing-Jing An; Dina Tabys; Yin-Dan Xie; Tian-Yu Zhao; Hao-Wei Ren; Ning Liu Journal: Int J Mol Sci Date: 2019-09-28 Impact factor: 5.923