Hua Guo1,2,3, Zhi-Lin Huang4, Wei Wang1,2,3, Shu-Xiao Zhang2,3, Juan Li5,2,3, Ke Cheng5,2,3, Ke Xu1,2,3, Yong He2,3, Si-Wen Gui5,2,3, Peng-Fei Li5,2,3, Hai-Yang Wang5,2,3, Zhi-Fang Dong4, Peng Xie1,5,2,3. 1. Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing, China. 2. Institute of Neuroscience and Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, China. 3. Chongqing Key Laboratory of Neurobiology, Chongqing, China. 4. Children's Hospital of Chongqing Medical University, Chongqing, China. 5. Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Abstract
PURPOSE: Major depressive disorder (MDD) is a worldwide concern and devastating psychiatric disease. The World Health Organization claims that MDD leads to at least 11.9% of the global burden of disease. However, the underlying pathophysiology mechanisms of MDD remain largely unknown. EXPERIMENTAL DESIGN: Herein, we proteomic-based strategy is used to compare the prefrontal cortex (PFC) in chronic social defeat stress (CSDS) model mice with a control group. Based on pooled samples, differential proteins are identified in the PFC proteome using iTRAQ coupled with LC-MS/MS. RESULTS: Ingenuity Pathway Analysis (IPA) is then followed to predict relevant pathways, with the ephrin receptor signaling pathway selected for further research. Additionally, as the selected key proteins of the ephrin receptor signaling pathway, ephrin type-B receptor 6 (EphB6) and the ERK pathway are validated by Western blotting. CONCLUSION AND CLINICAL RELEVANT: Altogether, increased understanding of the ephrin receptor signaling pathway in MDD is provided, which implicates further investigation of PFC dysfunction induced by CSDS treatment.
PURPOSE: Major depressive disorder (MDD) is a worldwide concern and devastating psychiatric disease. The World Health Organization claims that MDD leads to at least 11.9% of the global burden of disease. However, the underlying pathophysiology mechanisms of MDD remain largely unknown. EXPERIMENTAL DESIGN: Herein, we proteomic-based strategy is used to compare the prefrontal cortex (PFC) in chronic social defeat stress (CSDS) model mice with a control group. Based on pooled samples, differential proteins are identified in the PFC proteome using iTRAQ coupled with LC-MS/MS. RESULTS: Ingenuity Pathway Analysis (IPA) is then followed to predict relevant pathways, with the ephrin receptor signaling pathway selected for further research. Additionally, as the selected key proteins of the ephrin receptor signaling pathway, ephrin type-B receptor 6 (EphB6) and the ERK pathway are validated by Western blotting. CONCLUSION AND CLINICAL RELEVANT: Altogether, increased understanding of the ephrin receptor signaling pathway in MDD is provided, which implicates further investigation of PFC dysfunction induced by CSDS treatment.