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Literature DB >> 28966948

Labelling HaloTag Fusion Proteins with HaloTag Ligand in Living Cells.

Abstract

HaloTag has been widely used to label proteins in vitro and in vivo (Los et al., 2008). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging (Zhen et al., 2016).

Entities: Chemical

Keywords: Cbx; Epigenetics; HaloTag; Janelia Fluor™ dye; Live-cell single-molecule imaging; Polycomb

Year: 2017 PMID： 28966948 PMCID： PMC5621771 DOI： 10.21769/BioProtoc.2526

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

7 in total

1. HaloTag: a novel protein labeling technology for cell imaging and protein analysis.

Authors: Georgyi V Los; Lance P Encell; Mark G McDougall; Danette D Hartzell; Natasha Karassina; Chad Zimprich; Monika G Wood; Randy Learish; Rachel Friedman Ohana; Marjeta Urh; Dan Simpson; Jacqui Mendez; Kris Zimmerman; Paul Otto; Gediminas Vidugiris; Ji Zhu; Aldis Darzins; Dieter H Klaubert; Robert F Bulleit; Keith V Wood
Journal: ACS Chem Biol Date: 2008-06-20 Impact factor: 5.100

Review 2. Imaging live-cell dynamics and structure at the single-molecule level.

Authors: Zhe Liu; Luke D Lavis; Eric Betzig
Journal: Mol Cell Date: 2015-05-21 Impact factor: 17.970

Review 3. Dynamic chromatin technologies: from individual molecules to epigenomic regulation in cells.

Authors: Olivier Cuvier; Beat Fierz
Journal: Nat Rev Genet Date: 2017-05-22 Impact factor: 53.242

Review 4. Development of photostable fluorophores for molecular imaging.

Authors: Qinsi Zheng; Luke D Lavis
Journal: Curr Opin Chem Biol Date: 2017-05-23 Impact factor: 8.822

Review 5. Tracking single molecules at work in living cells.

Authors: Akihiro Kusumi; Taka A Tsunoyama; Kohichiro M Hirosawa; Rinshi S Kasai; Takahiro K Fujiwara
Journal: Nat Chem Biol Date: 2014-07 Impact factor: 15.040

6. A general method to improve fluorophores for live-cell and single-molecule microscopy.

Authors: Jonathan B Grimm; Brian P English; Jiji Chen; Joel P Slaughter; Zhengjian Zhang; Andrey Revyakin; Ronak Patel; John J Macklin; Davide Normanno; Robert H Singer; Timothée Lionnet; Luke D Lavis
Journal: Nat Methods Date: 2015-01-19 Impact factor: 28.547

7. Live-cell single-molecule tracking reveals co-recognition of H3K27me3 and DNA targets polycomb Cbx7-PRC1 to chromatin.

Authors: Chao Yu Zhen; Roubina Tatavosian; Thao Ngoc Huynh; Huy Nguyen Duc; Raibatak Das; Marko Kokotovic; Jonathan B Grimm; Luke D Lavis; Jun Lee; Frances J Mejia; Yang Li; Tingting Yao; Xiaojun Ren
Journal: Elife Date: 2016-10-10 Impact factor: 8.140

7 in total

4 in total

1. Nuclear condensates of the Polycomb protein chromobox 2 (CBX2) assemble through phase separation.

Authors: Roubina Tatavosian; Samantha Kent; Kyle Brown; Tingting Yao; Huy Nguyen Duc; Thao Ngoc Huynh; Chao Yu Zhen; Brian Ma; Haobin Wang; Xiaojun Ren
Journal: J Biol Chem Date: 2018-12-04 Impact factor: 5.157

Review 2. Advances in Chromatin Imaging at Kilobase-Scale Resolution.

Authors: Alistair Boettiger; Sedona Murphy
Journal: Trends Genet Date: 2020-01-29 Impact factor: 11.639

3. Live-cell single-molecule tracking reveals co-recognition of H3K27me3 and DNA targets polycomb Cbx7-PRC1 to chromatin.

4. CRISPR/Cas9 Genome Editing vs. Over-Expression for Fluorescent Extracellular Vesicle-Labeling: A Quantitative Analysis.

Authors: Karin Strohmeier; Martina Hofmann; Fabian Hauser; Dmitry Sivun; Sujitha Puthukodan; Andreas Karner; Georg Sandner; Pol-Edern Le Renard; Jaroslaw Jacak; Mario Mairhofer
Journal: Int J Mol Sci Date: 2021-12-28 Impact factor: 5.923

4 in total