Hyun-Ju Yu1, Ji-Ae Shin2, In-Hyoung Yang1, Dong-Hoon Won2, Chi Hyun Ahn2, Hye-Jeong Kwon2, Jeong-Sang Lee3, Nam-Pyo Cho1, Eun-Cheol Kim4, Hye-Jung Yoon2, Jae Il Lee2, Seong-Doo Hong2, Sung-Dae Cho5. 1. Department of Oral Pathology, School of Dentistry, and Institute of Oral Bioscience, Chonbuk National University, Jeonju 54896, Republic of Korea. 2. Department of Oral Pathology, School of Dentistry, and Dental Research Institute, Seoul National University, Seoul 03080, Republic of Korea. 3. Department of Functional Food and Biotechnology, College of Medical Science, Jeonju University, Jeonju 55069, Republic of Korea. 4. Department of Oral Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul, 02453, Republic of Korea. 5. Department of Oral Pathology, School of Dentistry, and Dental Research Institute, Seoul National University, Seoul 03080, Republic of Korea. Electronic address: efiwdsc@snu.ac.kr.
Abstract
OBJECTIVE: Caffeic acid phenethyl ester (CAPE), a natural honeybee product exhibits a spectrum of biological activities including antimicrobial, anti-inflammatory, antioxidant and antitumor actions. The purpose of this research was to investigate the anticancer potential of CAPE and its molecular mechanism in human oral cancer cell lines (YD15, HSC-4 and HN22 cells). DESIGN: To determine the apoptotic activity of CAPE and identify its molecular targets, trypan blue exclusion assay, soft agar assay, Western blot analysis, DAPI staining, and live/dead assay were performed. RESULTS: CAPE significantly suppressed transformation of neoplastic cells induced by epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol 13-acetate (TPA) without inhibiting growth. CAPE treatment inhibited cell growth, increased the cleavages of caspase-3 and poly (ADP-ribose) polymerase (PARP), and augmented the number of fragmented nuclei in human oral cancer cell lines. CAPE activated Bax protein causing it to undergo a conformational change, translocate to the mitochondrial outer membrane, and oligomere. CAPE also significantly increased Puma expression and interestingly Puma and Bax were co-localized. CONCLUSION: Overall, these results suggest that CAPE is a potent apoptosis-inducing agent in human oral cancer cell lines. Its action is accompanied by up-regulation of Bax and Puma proteins.
OBJECTIVE:Caffeic acid phenethyl ester (CAPE), a natural honeybee product exhibits a spectrum of biological activities including antimicrobial, anti-inflammatory, antioxidant and antitumor actions. The purpose of this research was to investigate the anticancer potential of CAPE and its molecular mechanism in humanoral cancer cell lines (YD15, HSC-4 and HN22 cells). DESIGN: To determine the apoptotic activity of CAPE and identify its molecular targets, trypan blue exclusion assay, soft agar assay, Western blot analysis, DAPI staining, and live/dead assay were performed. RESULTS:CAPE significantly suppressed transformation of neoplastic cells induced by epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol 13-acetate (TPA) without inhibiting growth. CAPE treatment inhibited cell growth, increased the cleavages of caspase-3 and poly (ADP-ribose) polymerase (PARP), and augmented the number of fragmented nuclei in humanoral cancer cell lines. CAPE activated Bax protein causing it to undergo a conformational change, translocate to the mitochondrial outer membrane, and oligomere. CAPE also significantly increased Puma expression and interestingly Puma and Bax were co-localized. CONCLUSION: Overall, these results suggest that CAPE is a potent apoptosis-inducing agent in humanoral cancer cell lines. Its action is accompanied by up-regulation of Bax and Puma proteins.
Authors: Anna Kleczka; Robert Kubina; Radosław Dzik; Krzysztof Jasik; Jerzy Stojko; Krzysztof Cholewa; Agata Kabała-Dzik Journal: Molecules Date: 2020-07-31 Impact factor: 4.411
Authors: Ana Paula C R Ferraz; Alessandra Sussulini; Jéssica L Garcia; Mariane R Costa; Fabiane V Francisqueti-Ferron; Artur J T Ferron; Carol Cristina V de A Silva; José Eduardo Corrente; Vanessa M Manfio; Vickeline Namba; Giuseppina P P Lima; Bismarque S Pereira; Denise Fecchio; Igor O Minatel; Klinsmann C Dos Santos; Camila R Corrêa Journal: Oxid Med Cell Longev Date: 2020-01-22 Impact factor: 6.543