Fatima Zohra Cherchali1, Mohamed Mouzali2, Jean Bernard Tommasino3, Dominique Decoret4, Nina Attik5, Hazem Aboulleil5, Dominique Seux6, Brigitte Grosgogeat6. 1. Laboratoire d'Etudes Physico-Chimiques des Matériaux, Application à l'Environnement (LEPCMAE), USTHB, Faculté de Chimie, Bab Ezzouar, Algérie; Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon, Université Lyon1, Villeurbanne, France. Electronic address: fatma-zohra.cherchali@univ-lyon1.fr. 2. Laboratoire d'Etudes Physico-Chimiques des Matériaux, Application à l'Environnement (LEPCMAE), USTHB, Faculté de Chimie, Bab Ezzouar, Algérie. 3. Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon, Université Lyon1, Villeurbanne, France. 4. UFR Odontologie, Université Lyon, Université Lyon1, Lyon, France. 5. Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon, Université Lyon1, Villeurbanne, France; UFR Odontologie, Université Lyon, Université Lyon1, Lyon, France. 6. Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon, Université Lyon1, Villeurbanne, France; UFR Odontologie, Université Lyon, Université Lyon1, Lyon, France; Service de Consultations et de Traitements Dentaires, Hospices Civils de Lyon, Lyon, France.
Abstract
OBJECTIVE: Development of antibacterial dental composites is the ultimate goal to decrease carious disease occurrence and increase the restoration longevity. For this purpose, the quaternary ammonium dimethyl-hexadecyl-methacryloxyethyl-ammonium iodide (DHMAI) and the methacryloyloxyethylphosphorylcholine (MPC) have been incorporated in experimental methacrylate-based composite resins. This aims to first investigate the effect of each alone and then their combined effect. METHODS: Synthesized DHMAI and commercial MPC were added either alone or combined at different concentrations to experimental dental composite. Flexural strength (FS) and modulus (FM) were tested to select the optimal concentrations. Only selected composites were evaluated for Vickers hardness (HV) and the degree of conversion (DC) using fourier transform infrared spectroscopy analysis (FTIR-ATR). Antibacterial activity was assessed using tests on colony-forming unit (CFU), scanning electron microscopy (SEM) and Alamarblue assay to measure the metabolic activity. Streptococcus mutans biofilm was chosen to be grown on the composite surfaces during 96h at 37°C. RESULTS: Incorporation of 7.5% DHMAI in composite improved the degree of conversion and gave a strong antibacterial effect with a reduction of (∼98%) in CFU and (∼50%) of metabolic activity with acceptable mechanical properties. Addition of MPC to DHMAI affects mechanical properties of composites without providing a better antibacterial activity. SIGNIFICANCE: Composites with DHMAI greatly reduced S. mutans biofilm and improved the degree of conversion without scarifying the composites' mechanical properties. DHMAI may have wide applicability to other dental materials in order to inhibit caries and improve the longevity of restorations.
OBJECTIVE: Development of antibacterial dental composites is the ultimate goal to decrease carious disease occurrence and increase the restoration longevity. For this purpose, the quaternary ammonium dimethyl-hexadecyl-methacryloxyethyl-ammonium iodide (DHMAI) and the methacryloyloxyethylphosphorylcholine (MPC) have been incorporated in experimental methacrylate-based composite resins. This aims to first investigate the effect of each alone and then their combined effect. METHODS: Synthesized DHMAI and commercial MPC were added either alone or combined at different concentrations to experimental dental composite. Flexural strength (FS) and modulus (FM) were tested to select the optimal concentrations. Only selected composites were evaluated for Vickers hardness (HV) and the degree of conversion (DC) using fourier transform infrared spectroscopy analysis (FTIR-ATR). Antibacterial activity was assessed using tests on colony-forming unit (CFU), scanning electron microscopy (SEM) and Alamarblue assay to measure the metabolic activity. Streptococcus mutans biofilm was chosen to be grown on the composite surfaces during 96h at 37°C. RESULTS: Incorporation of 7.5% DHMAI in composite improved the degree of conversion and gave a strong antibacterial effect with a reduction of (∼98%) in CFU and (∼50%) of metabolic activity with acceptable mechanical properties. Addition of MPC to DHMAI affects mechanical properties of composites without providing a better antibacterial activity. SIGNIFICANCE: Composites with DHMAI greatly reduced S. mutans biofilm and improved the degree of conversion without scarifying the composites' mechanical properties. DHMAI may have wide applicability to other dental materials in order to inhibit caries and improve the longevity of restorations.
Authors: Ana P Fugolin; Andreia B de Paula; Adam Dobson; Vincent Huynh; Rafael Consani; Jack L Ferracane; Carmem S Pfeifer Journal: Dent Mater Date: 2020-05-08 Impact factor: 5.304
Authors: Robert Stencel; Jacek Kasperski; Wojciech Pakieła; Anna Mertas; Elżbieta Bobela; Izabela Barszczewska-Rybarek; Grzegorz Chladek Journal: Materials (Basel) Date: 2018-06-18 Impact factor: 3.623