Shih-Jung Cheng1,2,3, Chi-Feng Chang4,5, Hui-Hsin Ko1,2, Jang-Jaer Lee1,2,3, Hsin-Ming Chen1,2,3, Huei-Jen Wang4, Hsiao-Shan Lin4, Chun-Pin Chiang1,2,3,6. 1. Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan. 2. Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan. 3. Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan. 4. iStat Biomedical Co, Ltd, New Taipei City, Taiwan. 5. Academia-Industry Bridging Program (AIBP), National Research Program for Bio-pharmaceuticals, Taipei, Taiwan. 6. Department of Dentistry, Far Eastern Memorial Hospital, New Taipei City, Taiwan.
Abstract
BACKGROUND: Effective biomarkers for oral cancer screening are important for early diagnosis and treatment of oral cancer. METHODS: Oral epithelial cell samples collected by mouth rinse were obtained from 65 normal control subjects, 108 patients with oral potentially malignant disorders, and 94 patients with oral squamous cell carcinoma (OSCC). Methylation levels of zinc-finger protein 582 (ZNF582) and paired-box 1 (PAX1) genes were quantified by real-time methylation-specific polymerase chain reaction after bisulfite conversion. RESULTS: An abrupt increase in methylated ZNF582 (ZNF582m ) and PAX1 (PAX1m ) levels and positive rates from mild dysplasia to moderate/severe dysplasia, indicating that both ZNF582m and PAX1m are effective biomarkers for differentiating moderate dysplasia or worse (MODY+) oral lesions. When ZNF582m /PAX1m tests were used for identifying MODY+ oral lesions, the sensitivity, specificity, and odds ratio (OR) were 0.65/0.64, 0.75/0.82, and 5.6/8.0, respectively. CONCLUSION: Hypermethylated ZNF582 and PAX1 genes in oral epithelial cells collected by mouth rinse are effective biomarkers for the detection of oral dysplasia and oral cancer.
BACKGROUND: Effective biomarkers for oral cancer screening are important for early diagnosis and treatment of oral cancer. METHODS: Oral epithelial cell samples collected by mouth rinse were obtained from 65 normal control subjects, 108 patients with oral potentially malignant disorders, and 94 patients with oral squamous cell carcinoma (OSCC). Methylation levels of zinc-finger protein 582 (ZNF582) and paired-box 1 (PAX1) genes were quantified by real-time methylation-specific polymerase chain reaction after bisulfite conversion. RESULTS: An abrupt increase in methylated ZNF582 (ZNF582m ) and PAX1 (PAX1m ) levels and positive rates from mild dysplasia to moderate/severe dysplasia, indicating that both ZNF582m and PAX1m are effective biomarkers for differentiating moderate dysplasia or worse (MODY+) oral lesions. When ZNF582m /PAX1m tests were used for identifying MODY+ oral lesions, the sensitivity, specificity, and odds ratio (OR) were 0.65/0.64, 0.75/0.82, and 5.6/8.0, respectively. CONCLUSION: Hypermethylated ZNF582 and PAX1 genes in oral epithelial cells collected by mouth rinse are effective biomarkers for the detection of oral dysplasia and oral cancer.
Authors: Óscar Rapado-González; José Luis López-Cedrún; Rafael López-López; Ana María Rodríguez-Ces; María Mercedes Suárez-Cunqueiro Journal: J Clin Med Date: 2021-04-29 Impact factor: 4.241