Helena Kovarikova1, Ivana Bubancova1, Jan Laco2, Katerina Sieglova2, Hana Vosmikova2, Milan Vosmik3, Pavel Dundr4, Kristyna Nemejcova4, Jaroslav Michalek5, Vladimir Palicka1, Marcela Chmelarova1. 1. Institute for Clinical Biochemistry and Diagnostics, Charles University, Faculty of Medicine, Hradec Kralove and University Hospital Hradec Kralove, Czech Republic. 2. The Fingerland Department of Pathology, Charles University, Faculty of Medicine, Hradec Kralove and University Hospital Hradec Kralove, Czech Republic. 3. Department of Oncology and Radiotherapy, Charles University, Faculty of Medicine, Hradec Kralove and University Hospital Hradec Kralove, Czech Republic. 4. Department of Pathology, Charles University, First Faculty of Medicine and General University Hospital in Prague, Czech Republic. 5. Department of Clinical and Molecular Pathology, Palacky University Olomouc, Faculty of Medicine and Dentistry and University Hospital Olomouc, Czech Republic.
Abstract
BACKGROUND: Tumors occurring in the sinonasal area are characterized by unfavorable outcome due to difficult diagnosis, treatment, and prognosis of the disease corresponding with the anatomic complexity of the area. METHODS: We used quantitative real-time polymerase chain reaction (PCR) to compare relative expression of miR-21, miR-141, and miR-200c in 70 formalin-fixed, paraffin-embedded samples of sinonasal carcinoma tissue (majority of squamous cell carcinoma [SCC] samples) with 17 control samples of sinonasal tissue. RESULTS: Our data showed significant upregulation of miR-21 in sinonasal cancer tissue. Expression levels of miR-141 and miR-200c were below detectable levels in both sinonasal cancer samples and healthy tissue. Kaplan-Meier analysis with log-rank survival showed that patients with SCC with high expression of miR-21 (highest quartile) had impaired survival close to reaching statistical significance (P = .0630). CONCLUSION: Our results suggest that miR-21 upregulation is involved in tumorigenesis of sinonasal carcinoma and that it is associated with poor prognosis. Thus, miR-21 could be used as a valuable prognostic biomarker.
BACKGROUND:Tumors occurring in the sinonasal area are characterized by unfavorable outcome due to difficult diagnosis, treatment, and prognosis of the disease corresponding with the anatomic complexity of the area. METHODS: We used quantitative real-time polymerase chain reaction (PCR) to compare relative expression of miR-21, miR-141, and miR-200c in 70 formalin-fixed, paraffin-embedded samples of sinonasal carcinoma tissue (majority of squamous cell carcinoma [SCC] samples) with 17 control samples of sinonasal tissue. RESULTS: Our data showed significant upregulation of miR-21 in sinonasal cancer tissue. Expression levels of miR-141 and miR-200c were below detectable levels in both sinonasal cancer samples and healthy tissue. Kaplan-Meier analysis with log-rank survival showed that patients with SCC with high expression of miR-21 (highest quartile) had impaired survival close to reaching statistical significance (P = .0630). CONCLUSION: Our results suggest that miR-21 upregulation is involved in tumorigenesis of sinonasal carcinoma and that it is associated with poor prognosis. Thus, miR-21 could be used as a valuable prognostic biomarker.
Authors: Jan Laco; Helena Kovaříková; Marcela Chmelařová; Hana Vošmiková; Kateřina Sieglová; Ivana Baranová; Pavel Dundr; Kristýna Němejcová; Jaroslav Michálek; Jana Šatanková; Milan Vošmik; Aleš Ryška Journal: Virchows Arch Date: 2018-01-11 Impact factor: 4.064
Authors: Petr Celakovsky; Helena Kovarikova; Viktor Chrobok; Jan Mejzlik; Jan Laco; Hana Vosmikova; Marcela Chmelarova; Ales Ryska Journal: In Vivo Date: 2021 Jan-Feb Impact factor: 2.155