Nicola J Rogers1, Hannah C Jeffery2, Sunil Claire3, David J Lewis1, Gerald Zikeli1, Nikolas J Hodges3,4, Stuart Egginton2,5, Gerard B Nash2,3, Zoe Pikramenou1,3. 1. School of Chemistry, University of Birmingham, Edgbaston, B15 2TT, UK. 2. Institute of Cardiovascular Sciences, University of Birmingham, Edgbaston, B15 2TT, UK. 3. Physical Sciences of Imaging in the Biological Sciences Doctoral Training Centre, University of Birmingham, Edgbaston, B15 2TT, UK. 4. School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, UK. 5. School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
Abstract
AIM: Imaging of blood flow in narrow channels and close to vessel walls is important in cardiovascular research for understanding pathogenesis. Our aim was to provide novel nanoprobes with visible emission and long lifetimes as trackers of flow. MATERIALS & METHODS: Gold nanoparticles coated with an iridium complex were prepared. Luminescence imaging was used to monitor their flows in different hematocrit blood and in murine tissues. RESULTS: The velocities are independent of hematocrit level and the nanoparticles entering blood circulation can be clearly detected in vessels in lungs, mesentery and the skeletal muscle. CONCLUSION: The work introduces for the first time iridium-based yellow-green luminescence with nanoparticle size of 100 nm for visualizing and monitoring flows with much higher resolution than conventional alternatives.
AIM: Imaging of blood flow in narrow channels and close to vessel walls is important in cardiovascular research for understanding pathogenesis. Our aim was to provide novel nanoprobes with visible emission and long lifetimes as trackers of flow. MATERIALS & METHODS: Gold nanoparticles coated with an iridium complex were prepared. Luminescence imaging was used to monitor their flows in different hematocrit blood and in murine tissues. RESULTS: The velocities are independent of hematocrit level and the nanoparticles entering blood circulation can be clearly detected in vessels in lungs, mesentery and the skeletal muscle. CONCLUSION: The work introduces for the first time iridium-based yellow-green luminescence with nanoparticle size of 100 nm for visualizing and monitoring flows with much higher resolution than conventional alternatives.
Entities:
Keywords:
blood flow; gold nanoparticles; imaging; luminescence; metal probes
Authors: Ana B Caballero; Lucia Cardo; Sunil Claire; James S Craig; Nikolas J Hodges; Anton Vladyka; Tim Albrecht; Luke A Rochford; Zoe Pikramenou; Michael J Hannon Journal: Chem Sci Date: 2019-08-13 Impact factor: 9.825