Patrícia Figueiredo1, Cláudio Ferro1,2, Marianna Kemell3, Zehua Liu1, Alexandros Kiriazis1, Kalle Lintinen4, Helena F Florindo2, Jari Yli-Kauhaluoma1, Jouni Hirvonen1, Mauri A Kostiainen4, Hélder A Santos1,5. 1. Drug Research Program, Division of Pharmaceutical Chemistry & Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland. 2. Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal. 3. Department of Chemistry, University of Helsinki, FI-00014 Helsinki, Finland. 4. Biohybrid Materials, Department of Bioproducts & Biosystems, Aalto University, FI-00076, Aalto, Finland. 5. Helsinki Institute of Life Science, HiLIFE, University of Helsinki, FI-00014 Helsinki, Finland.
Abstract
AIM: To carboxylate kraft lignin toward the functionalization of carboxylated lignin nanoparticles (CLNPs) with a block copolymer made of PEG, poly(histidine) and a cell-penetrating peptide and then evaluate the chemotherapeutic potential of the innovative nanoparticles. MATERIALS & METHODS: The produced nanoparticles were characterized and evaluated in vitro for stability and biocompatibility and the drug release profiles and antiproliferative effect were also assessed. RESULTS: The prepared CLNPs showed spherical shape and good size distribution, good stability in physiological media and low cytotoxicity in all the tested cell lines. A poorly water-soluble cytotoxic agent was successfully loaded into the CLNPs, improving its release profiles in a pH-sensitive manner and showing an enhanced antiproliferative effect in the different cancer cells compared with a normal endothelial cell line. CONCLUSION: The resulting CLNPs are promising candidates for anticancer therapy.
AIM: To carboxylate kraft lignin toward the functionalization of carboxylated lignin nanoparticles (CLNPs) with a block copolymer made of PEG, poly(histidine) and a cell-penetrating peptide and then evaluate the chemotherapeutic potential of the innovative nanoparticles. MATERIALS & METHODS: The produced nanoparticles were characterized and evaluated in vitro for stability and biocompatibility and the drug release profiles and antiproliferative effect were also assessed. RESULTS: The prepared CLNPs showed spherical shape and good size distribution, good stability in physiological media and low cytotoxicity in all the tested cell lines. A poorly water-soluble cytotoxic agent was successfully loaded into the CLNPs, improving its release profiles in a pH-sensitive manner and showing an enhanced antiproliferative effect in the different cancer cells compared with a normal endothelial cell line. CONCLUSION: The resulting CLNPs are promising candidates for anticancer therapy.
Authors: Patrícia Figueiredo; Mika H Sipponen; Kalle Lintinen; Alexandra Correia; Alexandros Kiriazis; Jari Yli-Kauhaluoma; Monika Österberg; Anne George; Jouni Hirvonen; Mauri A Kostiainen; Hélder A Santos Journal: Small Date: 2019-05-06 Impact factor: 13.281
Authors: Vimalkumar Balasubramanian; Andrea Poillucci; Alexandra Correia; Hongbo Zhang; Christian Celia; Hélder A Santos Journal: ACS Biomater Sci Eng Date: 2018-02-15