BACKGROUND: In Algeria, date palm is currently confronted to the Bayoud disease. Biotechnological tools such as protoplastsfusion can appear as an alternative to ensure rapid multiplication and improvement of this species. OBJECTIVES: Callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars. MATERIALS AND METHODS: Some factors influencing the isolation and culture of protoplasts segregated from the calli of three date palm (Phoenix dactylifera L.) cultivars (Deglet Nour, Akerbouch and Degla Beida) were studied. Protoplasts of each cultivar were cultured on a semi-solid medium supplemented with various hormonal balances. RESULTS: Maceration with an enzymatic solution containing 1.5% cellulase and 1% macerozyme R10 in the presence of 0.5 M mannitol for more than 16 h with gentle agitation allows isolation of a great number of viable protoplasts. In addition, purification of protoplasts on a cushion of 21 or 25% sucrose was effective in cell debris removal and maximum recovery. The culture of isolated protoplasts on a semi-solidified Murashige and Skoog medium, with 0.3% agarose, 2 mg. L-1 2,4-D and 0.5 mg.L-1 BAP allowed good viable protoplast maintenance as well as cell wall regeneration. After more than two months of culture, cell divisions were still occurring and microcalli became visible to the naked eye, containing a large number of cells. CONCLUSIONS: The developed protocol can be useful for application of somatic hybridization to improve date palm cultivars.
BACKGROUND: In Algeria, date palm is currently confronted to the Bayoud disease. Biotechnological tools such as protoplastsfusion can appear as an alternative to ensure rapid multiplication and improvement of this species. OBJECTIVES: Callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars. MATERIALS AND METHODS: Some factors influencing the isolation and culture of protoplasts segregated from the calli of three date palm (Phoenix dactylifera L.) cultivars (Deglet Nour, Akerbouch and Degla Beida) were studied. Protoplasts of each cultivar were cultured on a semi-solid medium supplemented with various hormonal balances. RESULTS: Maceration with an enzymatic solution containing 1.5% cellulase and 1% macerozyme R10 in the presence of 0.5 M mannitol for more than 16 h with gentle agitation allows isolation of a great number of viable protoplasts. In addition, purification of protoplasts on a cushion of 21 or 25% sucrose was effective in cell debris removal and maximum recovery. The culture of isolated protoplasts on a semi-solidified Murashige and Skoog medium, with 0.3% agarose, 2 mg. L-1 2,4-D and 0.5 mg.L-1 BAP allowed good viable protoplast maintenance as well as cell wall regeneration. After more than two months of culture, cell divisions were still occurring and microcalli became visible to the naked eye, containing a large number of cells. CONCLUSIONS: The developed protocol can be useful for application of somatic hybridization to improve date palm cultivars.
Authors: Abdelbasset El Hadrami; Abdelmalek El Idrissi-Tourane; Majida El Hassni; Fouad Daayf; Ismaïl El Hadrami Journal: C R Biol Date: 2005-08 Impact factor: 1.583
Authors: Ibrahim S Al-Mssallem; Songnian Hu; Xiaowei Zhang; Qiang Lin; Wanfei Liu; Jun Tan; Xiaoguang Yu; Jiucheng Liu; Linlin Pan; Tongwu Zhang; Yuxin Yin; Chengqi Xin; Hao Wu; Guangyu Zhang; Mohammed M Ba Abdullah; Dawei Huang; Yongjun Fang; Yasser O Alnakhli; Shangang Jia; An Yin; Eman M Alhuzimi; Burair A Alsaihati; Saad A Al-Owayyed; Duojun Zhao; Sun Zhang; Noha A Al-Otaibi; Gaoyuan Sun; Majed A Majrashi; Fusen Li; Jixiang Wang; Quanzheng Yun; Nafla A Alnassar; Lei Wang; Meng Yang; Rasha F Al-Jelaify; Kan Liu; Shenghan Gao; Kaifu Chen; Samiyah R Alkhaldi; Guiming Liu; Meng Zhang; Haiyan Guo; Jun Yu Journal: Nat Commun Date: 2013 Impact factor: 14.919